BMRB Entry 51535

Name: Backbone assignment for Functional Amyloid in pseudomonas FapA
Published: 2023-06-30

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51535

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignment for Functional Amyloid in pseudomonas FapA

Deposition date:

2022-07-28

Original release date:

2023-06-30

Authors:

Shin, Ben; Kumar, Amit; Xu, Yingqi; Matthews, Steve

Citation:

Citation: Ramussen, Helena; Kumar, Amit; Shin, Ben; Stylianou, Fisentzos; Sewell, Lee; Xu, Yingqi; Otzen, Daniel; Pedersen, Jan; Matthews, Steve. "FapA is an Intrinsically Disordered Chaperone for Pseudomonas Functional Amyloid FapC" J. Mol. Biol. 435, 167878-167878 (2023).
PubMed: 36368411

Assembly members:

Assembly members:
entity_1, polymer, 147 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Pseudomonas putida Taxonomy ID: 303 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas putida

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pRSF1b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGHHHHHHHDYDIPTTENLY FQGEDGIIVTGRNVQGFMVG RPSFGKDPYPSTANANPSQQ ILRATGGELSDNDFAGVSSG STITRAILPNGDLPGLSNTL GNNSAGLGAGAAAGHASGNS LGGQISGSIERGLAPLNNIG AMMGVQP

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	136
15N chemical shifts	90
1H chemical shifts	90

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FapA	1

Entities:

Entity 1, FapA 147 residues - Formula weight is not available

1

MET

GLY

HIS

ASP

2

TYR

ASP

ILE

PRO

THR

GLU

ASN

LEU

TYR

3

PHE

GLN

GLY

GLU

ASP

GLY

ILE

VAL

THR

4

GLY

ARG

ASN

VAL

GLN

GLY

PHE

MET

VAL

GLY

5

ARG

PRO

SER

PHE

GLY

LYS

ASP

PRO

TYR

PRO

6

SER

THR

ALA

ASN

ALA

ASN

PRO

SER

GLN

7

ILE

LEU

ARG

ALA

THR

GLY

GLU

LEU

SER

8

ASP

ASN

ASP

PHE

ALA

GLY

VAL

SER

GLY

9

SER

THR

ILE

THR

ARG

ALA

ILE

LEU

PRO

ASN

10

GLY

ASP

LEU

PRO

GLY

LEU

SER

ASN

THR

LEU

11

GLY

ASN

SER

ALA

GLY

LEU

GLY

ALA

GLY

12

ALA

GLY

HIS

ALA

SER

GLY

ASN

SER

13

LEU

GLY

GLN

ILE

SER

GLY

SER

ILE

GLU

14

ARG

GLY

LEU

ALA

PRO

LEU

ASN

ILE

GLY

15

ALA

MET

GLY

VAL

GLN

PRO

Samples:

sample_1: FapA, [U-98% 13C; U-98% 15N], 0.2 mM; sodium phosphate 20 mM

sample_conditions_1: ionic strength: 0.02 M; pH: 6.5; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 950 MHz
Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks