BMRB Entry 51522

Name: Region of Trak2 that interacts with Miro2 N-terminal GTPase
Published: 2022-08-22

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51522

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Region of Trak2 that interacts with Miro2 N-terminal GTPase

Deposition date:

2022-07-20

Original release date:

2022-08-22

Authors:

Smith, Cassandra; Jones, David

Citation:

Citation: Smith, Cassandra; Jones, David. "Understanding the molecular basis for the interaction of the mitochondrial associated protein Miro2 N-terminal GTPase with the trafficking protein TRAK2" .

Assembly members:

Assembly members:
entity_1, polymer, 84 residues, 9343 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-6P1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GPLGSGFMPEKLQIVKPLEG SQTLYHWQQLAQPNLGTILD PRPGVITKGFTQLPGDAIYH ISDLEEDEEEGITFQVQQPL EVEE

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	328
15N chemical shifts	74
1H chemical shifts	495

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Trak2	1

Entities:

Entity 1, Trak2 84 residues - 9343 Da.

The first four residues are non-native sequence from the affinity tag used for purification.

1

GLY

PRO

LEU

GLY

SER

GLY

PHE

MET

PRO

GLU

2

LYS

LEU

GLN

ILE

VAL

LYS

PRO

LEU

GLU

GLY

3

SER

GLN

THR

LEU

TYR

HIS

TRP

GLN

LEU

4

ALA

GLN

PRO

ASN

LEU

GLY

THR

ILE

LEU

ASP

5

PRO

ARG

PRO

GLY

VAL

ILE

THR

LYS

GLY

PHE

6

THR

GLN

LEU

PRO

GLY

ASP

ALA

ILE

TYR

HIS

7

ILE

SER

ASP

LEU

GLU

ASP

GLU

8

GLY

ILE

THR

PHE

GLN

VAL

GLN

PRO

LEU

9

GLU

VAL

GLU

Samples:

sample_1: Trak2, [U-98% 13C; U-98% 15N], 700 uM; HEPES 25 mM; sodium chloride 150 mM; magnesium chloride 1 mM; sucrose 5%; D2O 10%

sample_conditions_1: ionic strength: 150 mM; pH: 7.5; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
2D (HB)CB(CGCD)HD	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
1D 1H	sample_1	isotropic	sample_conditions_1

Software:

ANALYSIS v2.5.2 - chemical shift assignment

VNMRj v4.2 - collection

NMRPipe v10.9 - processing

NMR spectrometers:

Varian INOVA 600 MHz
Varian DD2 900 MHz

UNP	O60296
AlphaFold	Q9C0K6