BMRB Entry 51522

Title:
Region of Trak2 that interacts with Miro2 N-terminal GTPase
Deposition date:
2022-07-20
Original release date:
2022-08-22
Authors:
Smith, Cassandra; Jones, David
Citation:

Citation: Smith, Cassandra; Jones, David. "Understanding the molecular basis for the interaction of the mitochondrial associated protein Miro2 N-terminal GTPase with the trafficking protein TRAK2"  .

Assembly members:

Assembly members:
entity_1, polymer, 84 residues, 9343 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6P1

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts328
15N chemical shifts74
1H chemical shifts495

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Trak21

Entities:

Entity 1, Trak2 84 residues - 9343 Da.

The first four residues are non-native sequence from the affinity tag used for purification.

1   GLYPROLEUGLYSERGLYPHEMETPROGLU
2   LYSLEUGLNILEVALLYSPROLEUGLUGLY
3   SERGLNTHRLEUTYRHISTRPGLNGLNLEU
4   ALAGLNPROASNLEUGLYTHRILELEUASP
5   PROARGPROGLYVALILETHRLYSGLYPHE
6   THRGLNLEUPROGLYASPALAILETYRHIS
7   ILESERASPLEUGLUGLUASPGLUGLUGLU
8   GLYILETHRPHEGLNVALGLNGLNPROLEU
9   GLUVALGLUGLU

Samples:

sample_1: Trak2, [U-98% 13C; U-98% 15N], 700 uM; HEPES 25 mM; sodium chloride 150 mM; magnesium chloride 1 mM; sucrose 5%; D2O 10%

sample_conditions_1: ionic strength: 150 mM; pH: 7.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
2D (HB)CB(CGCD)HDsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
1D 1Hsample_1isotropicsample_conditions_1

Software:

ANALYSIS v2.5.2 - chemical shift assignment

VNMRj v4.2 - collection

NMRPipe v10.9 - processing

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian DD2 900 MHz

Related Database Links:

UNP O60296
AlphaFold Q9C0K6

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks