BMRB Entry 51516

Name: 1H, 13C, 15N NMR assignment of Engrailed2 homeodomain in anionic bicelles
Published: 2022-10-31

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51516

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

1H, 13C, 15N NMR assignment of Engrailed2 homeodomain in anionic bicelles

Deposition date:

2022-07-16

Original release date:

2022-10-31

Authors:

Carlier, Ludovic; LEQUIN, Olivier

Citation:

Citation: Carlier, Ludovic; Samson, Damien; Khemtemourian, Lucie; Joliot, Alain; Fuchs, Patrick; Lequin, Olivier. "Anionic lipids induce a fold-unfold transition in the membrane-translocating Engrailed homeodomain" Biochim. Biophys. Acta Biomembr. 1864, 184030-184030 (2022).
PubMed: 35988722

Assembly members:

Assembly members:
entity_1, polymer, 64 residues, Formula weight is not available

Natural source:

Natural source: Common Name: chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pSCherry

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GPASDKRPRTAFTAEQLQRL KAEFQTNRYLTEQRRQSLAQ ELGLNESQIKIWFQNKRAKI KKAT

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	176
15N chemical shifts	63
1H chemical shifts	241

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Engrailed 2 homeodomain	1

Entities:

Entity 1, Engrailed 2 homeodomain 64 residues - Formula weight is not available

Residues 200-259 of chicken Engrailed 2 homeodomain (UniProt Q05917) plus N-terminal extension GPAS.

1

GLY

PRO

ALA

SER

ASP

LYS

ARG

PRO

ARG

THR

2

ALA

PHE

THR

ALA

GLU

GLN

LEU

GLN

ARG

LEU

3

LYS

ALA

GLU

PHE

GLN

THR

ASN

ARG

TYR

LEU

4

THR

GLU

GLN

ARG

GLN

SER

LEU

ALA

GLN

5

GLU

LEU

GLY

LEU

ASN

GLU

SER

GLN

ILE

LYS

6

ILE

TRP

PHE

GLN

ASN

LYS

ARG

ALA

LYS

ILE

7

LYS

ALA

THR

Samples:

sample_1: Engrailed 2 homeodomain, [U-100% 13C; U-100% 15N], 1 mM; DHPC 50 mM; DMPG 25 mM; sodium phosphate 30 mM; sodium chloride 70 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe - processing

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 500 MHz