BMRB Entry 51516

Title:
1H, 13C, 15N NMR assignment of Engrailed2 homeodomain in anionic bicelles
Deposition date:
2022-07-16
Original release date:
2022-10-31
Authors:
Carlier, Ludovic; LEQUIN, Olivier
Citation:

Citation: Carlier, Ludovic; Samson, Damien; Khemtemourian, Lucie; Joliot, Alain; Fuchs, Patrick; Lequin, Olivier. "Anionic lipids induce a fold-unfold transition in the membrane-translocating Engrailed homeodomain"  Biochim. Biophys. Acta Biomembr. 1864, 184030-184030 (2022).
PubMed: 35988722

Assembly members:

Assembly members:
entity_1, polymer, 64 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pSCherry

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts176
15N chemical shifts63
1H chemical shifts241

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Engrailed 2 homeodomain1

Entities:

Entity 1, Engrailed 2 homeodomain 64 residues - Formula weight is not available

Residues 200-259 of chicken Engrailed 2 homeodomain (UniProt Q05917) plus N-terminal extension GPAS.

1   GLYPROALASERASPLYSARGPROARGTHR
2   ALAPHETHRALAGLUGLNLEUGLNARGLEU
3   LYSALAGLUPHEGLNTHRASNARGTYRLEU
4   THRGLUGLNARGARGGLNSERLEUALAGLN
5   GLULEUGLYLEUASNGLUSERGLNILELYS
6   ILETRPPHEGLNASNLYSARGALALYSILE
7   LYSLYSALATHR

Samples:

sample_1: Engrailed 2 homeodomain, [U-100% 13C; U-100% 15N], 1 mM; DHPC 50 mM; DMPG 25 mM; sodium phosphate 30 mM; sodium chloride 70 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

NMRPipe - processing

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 500 MHz

Related Database Links:

UniprotDB Q05917

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks