BMRB Entry 51514

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51514

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Title:
Backbone 1H, 13C and 15N chemical shift assignments, experimental 3JHN-HA scalar couplings, and 15N-relaxation rates of the C-terminal portion of human CHMP4C (residues 121-233)
Deposition date:
2022-07-11
Original release date:
2023-06-06
Authors:
Elias, Ruben; Deshmukh, Lalit
Citation:

Citation: Elias, Ruben; Zu, Yingqi; Su, Qi; Ghirlando, Rodolfo; Zhang, Jin; Deshmukh, Lalit. "Reversible phase separation of ESCRT protein ALIX through tyrosine phosphorylation"  Sci. Adv. 9, eadg3913-eadg3913 (2023).
PubMed: 37450591

Assembly members:

Assembly members:
entity_1, polymer, 115 residues, 12975 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSENMDLNKIDDLMQEITEQ QDIAQEISEAFSQRVGFGDD FDEDELMAELEELEQEELNK KMTNIRLPNVPSCSLPAQPN RKPGMSSTARRSRAASSQRA EEEDDDIKQLAAWAT

Data sets:
Data typeCount
13C chemical shifts429
15N chemical shifts105
1H chemical shifts375
T1 relaxation values101
T2 relaxation values101
coupling constants100

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Human CHMP4C1

Entities:

Entity 1, Human CHMP4C 115 residues - 12975 Da.

1   GLYSERGLUASNMETASPLEUASNLYSILE
2   ASPASPLEUMETGLNGLUILETHRGLUGLN
3   GLNASPILEALAGLNGLUILESERGLUALA
4   PHESERGLNARGVALGLYPHEGLYASPASP
5   PHEASPGLUASPGLULEUMETALAGLULEU
6   GLUGLULEUGLUGLNGLUGLULEUASNLYS
7   LYSMETTHRASNILEARGLEUPROASNVAL
8   PROSERCYSSERLEUPROALAGLNPROASN
9   ARGLYSPROGLYMETSERSERTHRALAARG
10   ARGSERARGALAALASERSERGLNARGALA
11   GLUGLUGLUASPASPASPILELYSGLNLEU
12   ALAALATRPALATHR

Related Database Links:

UNP Q96CF2
AlphaFold B2RBZ1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks