BMRB Entry 51512

Name: Backbone 1H, 13C, and 15N chemical shift assignments for the chromoshadow domain (residue 112-176) of human heterochromatin protein 1a (HP1a).
Published: 2023-06-30

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51512

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Backbone 1H, 13C, and 15N chemical shift assignments for the chromoshadow domain (residue 112-176) of human heterochromatin protein 1a (HP1a).

Deposition date:

2022-07-08

Original release date:

2023-06-30

Authors:

Her, Cheenou; Debelouchina, Galia

Citation:

Citation: Her, Cheenou; Phan, Tien; Jovic, Nina; Kapoor, Utkarsh; Ackermann, Bryce; Rizuan, Azamat; Kim, Young; Mittal, Jeetain; Debelouchina, Galia. "Molecular interactions underlying the phase separation of HP1a: role of phosphorylation, ligand and nucleic acid binding" Nucleic Acids Res. 50, 12702-12722 (2022).
PubMed: 36537242

Assembly members:

Assembly members:
entity_1, polymer, 66 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET (2B-T)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SDIARGFERGLEPEKIIGAT DSCGDLMFLMKWKDTDEADL VLAKEANVKCPQIVIAFYEE RLTWHA

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	126
15N chemical shifts	62
1H chemical shifts	62

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HP1a-CSD	1

Entities:

Entity 1, HP1a-CSD 66 residues - Formula weight is not available

1

SER

ASP

ILE

ALA

ARG

GLY

PHE

GLU

ARG

GLY

2

LEU

GLU

PRO

GLU

LYS

ILE

GLY

ALA

THR

3

ASP

SER

CYS

GLY

ASP

LEU

MET

PHE

LEU

MET

4

LYS

TRP

LYS

ASP

THR

ASP

GLU

ALA

ASP

LEU

5

VAL

LEU

ALA

LYS

GLU

ALA

ASN

VAL

LYS

CYS

6

PRO

GLN

ILE

VAL

ILE

ALA

PHE

TYR

GLU

7

ARG

LEU

THR

TRP

HIS

ALA

Samples:

sample_1: potassium chloride 75 mM; TCEP 1 mM; HEPES 20 mM; sodium azide 0.05%; Chromoshadow domain of human heterochromatin protein 1a (HP1a), [U-99% 13C; U-99% 15N], 400 uM

sample_conditions_1: pH: 7.2; pressure: 1 atm; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

SPARKY - chemical shift assignment

NMR spectrometers:

Bruker AVANCE NEO 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks