BMRB Entry 5151

Title:
Designed Protein G Core Variants Fold to Native-like Structures: Sequence Selection by ORBIT Tolerates Variation in Backbone Specification
Deposition date:
2001-09-13
Original release date:
2001-10-17
Authors:
Ross, S.; Sarisky, C.; Su, A.; Mayo, S.
Citation:

Citation: Ross, S.; Sarisky, C.; Su, A.; Mayo, S.. "Designed Protein G Core Variants Fold to Native-like Structures: Sequence Selection by ORBIT Tolerates Variation in Backbone Specification"  Protein Sci. 10, 450-454 (2001).
PubMed: 11266631

Assembly members:

Assembly members:
IMMUNOGLOBULIN G BINDING PROTEIN G, polymer, 57 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Streptococcus sp.   Taxonomy ID: 1306   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Streptococcus sp.

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
IMMUNOGLOBULIN G BINDING PROTEIN G: MTTFKLIINGKTLKGETTTE AVDAATAEKVFKQYANDNGI DGEWTYDDATKTFTVTE

Data sets:
Data typeCount
1H chemical shifts378

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1immunoglobulin G binding B1 domain of protein G1

Entities:

Entity 1, immunoglobulin G binding B1 domain of protein G 57 residues - Formula weight is not available

1   METTHRTHRPHELYSLEUILEILEASNGLY
2   LYSTHRLEULYSGLYGLUTHRTHRTHRGLU
3   ALAVALASPALAALATHRALAGLULYSVAL
4   PHELYSGLNTYRALAASNASPASNGLYILE
5   ASPGLYGLUTRPTHRTYRASPASPALATHR
6   LYSTHRPHETHRVALTHRGLU

Samples:

sample_1: IMMUNOGLOBULIN G BINDING PROTEIN G 3 mM; sodium phosphate 50 mM; H2O 90%; D2O 10%

sample_2: IMMUNOGLOBULIN G BINDING PROTEIN G 3 mM; sodium phosphate 50 mM; D2O 100%

sample_cond_1: ionic strength: 50 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available
E-COSYnot availablenot availablenot available
TOCSYnot availablenot availablenot available

Software:

VNMR v5.2f - collection

VNMR v5.3b - collection

VNMR v6.1b - collection

ANSIG v3.3 - processing

X-PLOR v3.1 - refinement, structure solution

NMR spectrometers:

  • Varian UNITYplus 600 MHz

Related Database Links:

PDB