BMRB Entry 51505

Title:
Backbone 1H, 13C and 15N Assignments of MAX(1-21)
Deposition date:
2022-06-28
Original release date:
2022-10-24
Authors:
Schutz, Stefan; Jahnke, Wolfgang
Citation:

Citation: Schutz, Stefan; Bergsdorf, Christian; Goretzki, Benedikt; Lingel, Andreas; Renatus, Martin; Gossert, Alvar; Jahnke, Wolfgang. "The disordered MAX N-terminus modulates DNA binding of the transcription factor MYC:MAX"  J. Mol. Biol. 434, 167833-167833 (2022).
PubMed: 36174765

Assembly members:

Assembly members:
entity_1, polymer, 21 residues, 2434.4 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETM41-like

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSDNDDIEVESDEEQPRFQS A

Data sets:
Data typeCount
13C chemical shifts87
15N chemical shifts24
1H chemical shifts24

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MAX(1-21), trans1
2MAX(1-21), cis1

Entities:

Entity 1, MAX(1-21), trans 21 residues - 2434.4 Da.

1   METSERASPASNASPASPILEGLUVALGLU
2   SERASPGLUGLUGLNPROARGPHEGLNSER
3   ALA

Samples:

sample_1: MAX(1-21), [U-99% 13C; U-99% 15N], 1.05 mM; D2O, [U-99% 2H], 5 v/v; HEPES 50 mM; sodium chloride 100 mM; TCEP 2 mM; magnesium chloride 20 mM; EDTA 20 mM; ATP 5 mM

sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D CBCANHsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.2 - collection, data analysis, processing

CcpNMR v3.0 - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks