BMRB Entry 51502

Title:
1H, 13C, and 15N chemical shift assignments of MqsR in complex with its canonical antitoxin MqsA (Residues 1-76)
Deposition date:
2022-06-21
Original release date:
2022-10-31
Authors:
Yu, Victor; Ronzone, Erik; Peti, Wolfgang; Page, Rebecca
Citation:

Citation: Yu, Victor; Ronzone, Eric; Lord, Dana; Peti, Wolfgang; Page, Rebecca. "MqsR is a noncanonical microbial RNase toxin that is inhibited by antitoxin MqsA via steric blockage of substrate binding"  J. Biol. Chem. 298, 102535-102535 (2022).
PubMed: 36162504

Assembly members:

Assembly members:
entity_1, polymer, 98 residues, Formula weight is not available
entity_2, polymer, 86 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET30a

Data sets:
Data typeCount
13C chemical shifts88
15N chemical shifts88
1H chemical shifts88

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MqsR1
2MqsA2

Entities:

Entity 1, MqsR 98 residues - Formula weight is not available

1   METGLULYSARGTHRPROHISTHRARGLEU
2   SERGLNVALLYSLYSLEUVALASNALAGLY
3   GLNVALARGTHRTHRARGSERALALEULEU
4   ASNALAASPGLULEUGLYLEUASPPHEASP
5   GLYMETCYSASNVALILEILEGLYLEUSER
6   GLUSERASPPHETYRLYSSERMETTHRTHR
7   TYRSERASPHISTHRILETRPGLNASPVAL
8   TYRARGPROARGLEUVALTHRGLYGLNVAL
9   TYRLEULYSILETHRVALILEHISASPVAL
10   LEUILEVALSERPHELYSGLULYS

Entity 2, MqsA 86 residues - Formula weight is not available

MAHHHHHHAA purification tag and cloning artifact

1   METALAHISHISHISHISHISHISALAALA
2   METLYSCYSPROVALCYSHISGLNGLYGLU
3   METVALSERGLYILELYSASPILEPROTYR
4   THRPHEARGGLYARGLYSTHRVALLEULYS
5   GLYILEHISGLYLEUTYRCYSVALHISCYS
6   GLUGLUSERILEMETASNLYSGLUGLUSER
7   ASPALAPHEMETALAGLNVALLYSALAPHE
8   ARGALASERVALASNALAGLUTHRVALALA
9   PROGLUPHEILEVALLYS

Samples:

sample_1: sodium chloride 50 mM; sodium phosphate 20 mM; TCEP 0.5 mM; MqsR, [U-99% 13C; U-99% 15N], 150 uM; MqsA 150 uM

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

CARA v1.9 - chemical shift assignment

TOPSPIN v4.1.1 - collection, processing

NMR spectrometers:

  • Bruker Avance 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks