BMRB Entry 51494

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for TDP-43 low complexity domain with methionine sulfoxide modifications
Published: 2023-01-30

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51494

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for TDP-43 low complexity domain with methionine sulfoxide modifications

Deposition date:

2022-06-16

Original release date:

2023-01-30

Authors:

Carrasco, Jaime; Anton, Rosa; Pantoja-Uceda, David; Laurents, Douglas; Oroz, Javier

Citation:

Citation: Carrasco, Jaime; Anton, Rosa; Valbuena, Alejandro; Pantoja-Uceda, David; Mukhi, Mayur; Hervas, Ruben; Laurents, Douglas; Gasset, Maria; Oroz, Javier. "Metamorphism in TDP-43 prion-like domain determines chaperone recognition" Nat. Commun. 14, 466-466 (2023).
PubMed: 36709343

Assembly members:

Assembly members:
entity_1, polymer, 141 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GRFGGNPGGFGNQGGFGNSR GGGAGLGNNQGSNXGGGXNF GAFSINPAXXAAAQAALQSS WGXXGXLASQQNQSGPSGNN QNQGNXQREPNQAFGSGNNS YSGSNSGAAIGWGSASNAGS GSGFNGGFGSSXDSKSSGWG X

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	351
15N chemical shifts	136
1H chemical shifts	457

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	TDP-43 PLD	1

Entities:

Entity 1, TDP-43 PLD 141 residues - Formula weight is not available

1

GLY

ARG

PHE

GLY

ASN

PRO

GLY

PHE

2

GLY

ASN

GLN

GLY

PHE

GLY

ASN

SER

ARG

3

GLY

ALA

GLY

LEU

GLY

ASN

GLN

4

GLY

SER

ASN

MHO

GLY

MHO

ASN

PHE

5

GLY

ALA

PHE

SER

ILE

ASN

PRO

ALA

MHO

6

ALA

GLN

ALA

LEU

GLN

SER

7

TRP

GLY

MHO

GLY

MHO

LEU

ALA

SER

GLN

8

GLN

ASN

GLN

SER

GLY

PRO

SER

GLY

ASN

9

GLN

ASN

GLN

GLY

ASN

MHO

GLN

ARG

GLU

PRO

10

ASN

GLN

ALA

PHE

GLY

SER

GLY

ASN

SER

11

TYR

SER

GLY

SER

ASN

SER

GLY

ALA

ILE

12

GLY

TRP

GLY

SER

ALA

SER

ASN

ALA

GLY

SER

13

GLY

SER

GLY

PHE

ASN

GLY

PHE

GLY

SER

14

SER

MHO

ASP

SER

LYS

SER

GLY

TRP

GLY

15

MHO

Samples:

sample_1: TDP-43 PLD, [U-98% 13C; U-98% 15N], 0.3 mM; HEPES 20 mM; KCL 10 mM; MgCl2 5 mM; ATP 1 mM; DTT 1 mM; PMSF 0.2 mM; sodium azide 0.03%; DSS 0.5 mM

sample_conditions_1: ionic strength: 0.01 M; pH: 6.8; pressure: 1 atm; temperature: 288 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

SPARKY v3.190 - chemical shift assignment

NMR spectrometers:

Bruker AVANCE NEO 800 MHz

UNP	Q13148
AlphaFold	Q96DJ0