BMRB Entry 51492

Title:
NTD TDP-43 with 3% SB3-10   PubMed: 35807552
Deposition date:
2022-06-14
Original release date:
2022-08-08
Authors:
Cantarutti, Cristina; Moretti, Matteo; Corazza, Alessandra; Chiti, Fabrizio
Citation:

Citation: Moretti, Matteo; Marzi, Isabella; Cantarutti, Cristina; Vivoli Vega, Mirella; Mandaliti, Walter; Mimmi, Maria Chiara; Bemporad, Francesco; Corazza, Alessandra; Chiti, Fabrizio. "Conversion of the Native N-Terminal Domain of TDP-43 into a Monomeric Alternative Fold with Lower Aggregation Propensity"  Molecules 27, 4309-4309 (2022).

Assembly members:

Assembly members:
entity_1, polymer, 77 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pNIC28-Bsa4

Experimental source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pNIC28-Bsa4

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts390
15N chemical shifts129
1H chemical shifts129

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TDP-43, form A1
2TDP-43, form B1

Entities:

Entity 1, TDP-43, form A 77 residues - Formula weight is not available

1   METSERGLUTYRILEARGVALTHRGLUASP
2   GLUASNASPGLUPROILEGLUILEPROSER
3   GLUASPASPGLYTHRVALLEULEUSERTHR
4   VALTHRALAGLNPHEPROGLYALACYSGLY
5   LEUARGTYRARGASNPROVALSERGLNCYS
6   METARGGLYVALARGLEUVALGLUGLYILE
7   LEUHISALAPROASPALAGLYTRPGLYASN
8   LEUVALTYRVALVALASNTYR

Samples:

sample_1: NTD TDP-43, [U-100% 13C; U-100% 15N], 250 uM; sulfobetaine 3-10 3%; D2O, [U-100% 2H], 5%; sodium phosphate 5 mM; NaCl 50 mM; DTT 1 mM; TSP 10 uM

sample_conditions_1: ionic strength: 0.08 M; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection, processing

NMRFAM-SPARKY - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker AVANCE NEO 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks