BMRB Entry 51492

Name: NTD TDP-43 with 3% SB3-10
Published: 2022-08-08

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51492

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NTD TDP-43 with 3% SB3-10

Deposition date:

2022-06-14

Original release date:

2022-08-08

Authors:

Cantarutti, Cristina; Moretti, Matteo; Corazza, Alessandra; Chiti, Fabrizio

Citation:

Citation: Moretti, Matteo; Marzi, Isabella; Cantarutti, Cristina; Vivoli Vega, Mirella; Mandaliti, Walter; Mimmi, Maria Chiara; Bemporad, Francesco; Corazza, Alessandra; Chiti, Fabrizio. "Conversion of the Native N-Terminal Domain of TDP-43 into a Monomeric Alternative Fold with Lower Aggregation Propensity" Molecules 27, 4309-4309 (2022).
PubMed: 35807552

Assembly members:

Assembly members:
entity_1, polymer, 77 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pNIC28-Bsa4

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSEYIRVTEDENDEPIEIPS EDDGTVLLSTVTAQFPGACG LRYRNPVSQCMRGVRLVEGI LHAPDAGWGNLVYVVNY

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
- assigned_chemical_shifts_2

Data type	Count
13C chemical shifts	390
15N chemical shifts	129
1H chemical shifts	129

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	TDP-43, form A	1
2	TDP-43, form B	1

Entities:

Entity 1, TDP-43, form A 77 residues - Formula weight is not available

1

MET

SER

GLU

TYR

ILE

ARG

VAL

THR

GLU

ASP

2

GLU

ASN

ASP

GLU

PRO

ILE

GLU

ILE

PRO

SER

3

GLU

ASP

GLY

THR

VAL

LEU

SER

THR

4

VAL

THR

ALA

GLN

PHE

PRO

GLY

ALA

CYS

GLY

5

LEU

ARG

TYR

ARG

ASN

PRO

VAL

SER

GLN

CYS

6

MET

ARG

GLY

VAL

ARG

LEU

VAL

GLU

GLY

ILE

7

LEU

HIS

ALA

PRO

ASP

ALA

GLY

TRP

GLY

ASN

8

LEU

VAL

TYR

VAL

ASN

TYR

Samples:

sample_1: NTD TDP-43, [U-100% 13C; U-100% 15N], 250 uM; sulfobetaine 3-10 3%; D2O, [U-100% 2H], 5%; sodium phosphate 5 mM; NaCl 50 mM; DTT 1 mM; TSP 10 uM

sample_conditions_1: ionic strength: 0.08 M; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection, processing

NMRFAM-SPARKY - chemical shift assignment, peak picking

NMR spectrometers:

Bruker AVANCE NEO 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks