BMRB Entry 51490

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51490

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Title:
UNC-89 SH3 domain (residues 61-128)
Deposition date:
2022-06-14
Original release date:
2023-07-13
Authors:
Mateos, Borja; Sanfeliu-Cerdan, Neus; Salvatella, Xavier; Krieg, Michael
Citation:

Citation: Sanfeliu-Cerdan, Neus; Mateos, Borja; Garcia-Cabau, Carla; Catala-Castro, Frederic; Ribera, Maria; Porta-de-la-Riva, Montserrat; Wieser, Stefan; Salvatella, Xavier; Krieg, Michael. "A viscoelastic switch of Stomatin condensatesgoverns transport and mechanotransduction"  Nat. Cell Biol. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 70 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GPESRSYPVYIAIQDYTPDK EDVEAIPLEQGQIVEVLDKK NSVRWLVRTKARPPRSGWVP GSYFETPTEF

Data sets:
Data typeCount
13C chemical shifts200
15N chemical shifts61
1H chemical shifts61

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1UNC89 SH3 domain1

Entities:

Entity 1, UNC89 SH3 domain 70 residues - Formula weight is not available

1   GLYPROGLUSERARGSERTYRPROVALTYR
2   ILEALAILEGLNASPTYRTHRPROASPLYS
3   GLUASPVALGLUALAILEPROLEUGLUGLN
4   GLYGLNILEVALGLUVALLEUASPLYSLYS
5   ASNSERVALARGTRPLEUVALARGTHRLYS
6   ALAARGPROPROARGSERGLYTRPVALPRO
7   GLYSERTYRPHEGLUTHRPROTHRGLUPHE

Samples:

sample_1: UNC89 SH3 domain, [U-99% 13C; U-99% 15N], 1.25 mM; D2O, [U-2H], 10%; DSS 10 uM; TCEP 1 mM

sample_conditions_1: ionic strength: 0 M; pH: 7.4; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

NMRPipe - processing

TOPSPIN - collection

MddNMR - processing

CcpNMR - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE NEO 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks