BMRB Entry 51487

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51487

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Title:
Chemical Shift Assignments RECQ4(322-400)
Deposition date:
2022-06-10
Original release date:
2023-10-27
Authors:
Papageorgiou, Anna; Tripsianes, Konstantinos
Citation:

Citation: Papageorgiou, Anna; Pospisilova, Michaela; Cibulka, Jakub; Ashraf, Raghib; Waudby, Christopher; Kaderavek, Pavel; Maroz, Volha; Kubicek, Karel; Prokop, Zbynek; Krejci, Lumir; Tripsianes, Konstantinos. "Recognition and coacervation of G-quadruplexes by a multifunctional disordered region in RECQ4 helicase"  Nat. Commun. 14, 6751-6751 (2023).
PubMed: 37875529

Assembly members:

Assembly members:
entity_1, polymer, 79 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-M11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: LSPSSQARAGKAEGTAPLHI FPRLARHDRGNYVRLNMKQK HYVRGRALRSRLLRKQAWKQ KWRKKGECFGGGGATVTTK

Data sets:
Data typeCount
13C chemical shifts299
15N chemical shifts73
1H chemical shifts436

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RECQ41

Entities:

Entity 1, RECQ4 79 residues - Formula weight is not available

1   LEUSERPROSERSERGLNALAARGALAGLY
2   LYSALAGLUGLYTHRALAPROLEUHISILE
3   PHEPROARGLEUALAARGHISASPARGGLY
4   ASNTYRVALARGLEUASNMETLYSGLNLYS
5   HISTYRVALARGGLYARGALALEUARGSER
6   ARGLEULEUARGLYSGLNALATRPLYSGLN
7   LYSTRPARGLYSLYSGLYGLUCYSPHEGLY
8   GLYGLYGLYALATHRVALTHRTHRLYS

Samples:

sample_1: RECQ4 322-400, [U-99% 13C; U-99% 15N], 0.7 mM; NaCl 100 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1
4D HCNH TOCSYsample_1isotropicsample_conditions_1

Software:

SPARKY - data analysis

TOPSPIN - collection

SSA - processing

NMR spectrometers:

  • Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks