BMRB Entry 51486
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51486
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NMR-STAR v3 text file.
XML gzip file.
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Citation: Clements, Christopher; Vogeli, Beat; Shellman, Yiqun; Henen, Morkos. "SAM1 domain in SASH1 is showing two solution species among them one is monomeric disordered and the other is folded oligomeric." J. Struct. Biol. 214, 107914-107914 (2022).
PubMed: 36341956
Assembly members:
entity_1, polymer, 82 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pMAL-c4x-1-H
Entity Sequences (FASTA):
entity_1: SKGRPPQPKSVEDLLDRINL
KEHMPTFLFNGYEDLDTFKL
LEEEDLDELNIRDPEHRAVL
LTAVELLQEYDSNSDQHHHH
HH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 192 |
| 15N chemical shifts | 57 |
| 1H chemical shifts | 57 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SAM1-SASH1 | 1 |
Entities:
Entity 1, SAM1-SASH1 82 residues - Formula weight is not available
| 1 | SER | LYS | GLY | ARG | PRO | PRO | GLN | PRO | LYS | SER | ||||
| 2 | VAL | GLU | ASP | LEU | LEU | ASP | ARG | ILE | ASN | LEU | ||||
| 3 | LYS | GLU | HIS | MET | PRO | THR | PHE | LEU | PHE | ASN | ||||
| 4 | GLY | TYR | GLU | ASP | LEU | ASP | THR | PHE | LYS | LEU | ||||
| 5 | LEU | GLU | GLU | GLU | ASP | LEU | ASP | GLU | LEU | ASN | ||||
| 6 | ILE | ARG | ASP | PRO | GLU | HIS | ARG | ALA | VAL | LEU | ||||
| 7 | LEU | THR | ALA | VAL | GLU | LEU | LEU | GLN | GLU | TYR | ||||
| 8 | ASP | SER | ASN | SER | ASP | GLN | HIS | HIS | HIS | HIS | ||||
| 9 | HIS | HIS |
Samples:
sample_1: SAM1-SASH1, [U-100% 13C; U-100% 15N], 345.7 uM; NaCl 100 mM
sample_conditions_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 278 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR - chemical shift assignment, data analysis, peak picking
VNMRj - collection
NMRPipe - data analysis, peak picking, processing
SPARKY - data analysis
NMR spectrometers:
- Varian INOVA 900 MHz
- Bruker AVANCE NEO 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks