BMRB Entry 51485

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51485

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Title:
Chemical shift assignments of the C-terminal domain of AKAP5 (AKAP79), residues 300-427
Deposition date:
2022-06-09
Original release date:
2022-06-14
Authors:
Watson, Matthew; Almeida, Teresa; Ray, Arundhati; Hanack, Christina; Elston, Rory; Btesh, Joan; McNaughton, Peter; Stott, Katherine
Citation:

Citation: Watson, Matthew; Almeida, Teresa; Ray, Arundhati; Hanack, Christina; Elston, Rory; Btesh, Joan; McNaughton, Peter; Stott, Katherine. "Hidden Multivalency in Phosphatase Recruitment by a Disordered AKAP Scaffold"  J. Mol. Biol. 434, 167682-167682 (2022).
PubMed: 35697294

Assembly members:

Assembly members:
entity_1, polymer, 130 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pHAT3 AKAP5c

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSVAEETKPKDTELSQESDF KENGITEEKSKSEESKRMEP IAIIITDTEISEFDVTKSKN VPKQFLISAENEQVGVFAND NGFEDRTSEQYETLLIETAS SLVKNAIQLSIEQLVNEMAS DDNKINNLLQ

Data sets:
Data typeCount
15N chemical shifts125
1H chemical shifts125

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AKAP5 (AKAP79)1

Entities:

Entity 1, AKAP5 (AKAP79) 130 residues - Formula weight is not available

1   GLYSERVALALAGLUGLUTHRLYSPROLYS
2   ASPTHRGLULEUSERGLNGLUSERASPPHE
3   LYSGLUASNGLYILETHRGLUGLULYSSER
4   LYSSERGLUGLUSERLYSARGMETGLUPRO
5   ILEALAILEILEILETHRASPTHRGLUILE
6   SERGLUPHEASPVALTHRLYSSERLYSASN
7   VALPROLYSGLNPHELEUILESERALAGLU
8   ASNGLUGLNVALGLYVALPHEALAASNASP
9   ASNGLYPHEGLUASPARGTHRSERGLUGLN
10   TYRGLUTHRLEULEUILEGLUTHRALASER
11   SERLEUVALLYSASNALAILEGLNLEUSER
12   ILEGLUGLNLEUVALASNGLUMETALASER
13   ASPASPASNLYSILEASNASNLEULEUGLN

Samples:

sample_1: AKAP5 (AKAP79), [U-98% 15N], 50-100 uM; PIPES 10 mM; NaCl 150 mM

sample_conditions_1: ionic strength: 0.16 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

AZARA v2.7 - processing

ANALYSIS v2.4 - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks