BMRB Entry 51483

Name: Solid-state NMR assignment of fibrils formed by tau(297-391)
Published: 2023-01-30

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51483

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solid-state NMR assignment of fibrils formed by tau(297-391)

Deposition date:

2022-06-08

Original release date:

2023-01-30

Authors:

Siemer, Ansgar; Serpell, Louise

Citation:

Citation: Al-Hilaly, Youssra; Hurd, Connor; Rickard, Janet; Harrington, Charles; Storey, John; Wischik, Claude; Serpell, Louise; Siemer, Ansgar. "Solid-state NMR of paired helical filaments formed by the core tau fragment tau(297-391)" Front. Neurosci. 16, 988074-988074 (2022).
PubMed: 36570831

Assembly members:

Assembly members:
entity_1, polymer, 95 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pRK172

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: IKHVPGGGSVQIVYKPVDLS KVTSKCGSLGNIHHKPGGGQ VEVKSEKLDFKDRVQSKIGS LDNITHVPGGGNKKIETHKL TFRENAKAKTDHGAE

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	160
15N chemical shifts	36

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	tau(297-391)	1

Entities:

Entity 1, tau(297-391) 95 residues - Formula weight is not available

1

ILE

LYS

HIS

VAL

PRO

GLY

SER

VAL

2

GLN

ILE

VAL

TYR

LYS

PRO

VAL

ASP

LEU

SER

3

LYS

VAL

THR

SER

LYS

CYS

GLY

SER

LEU

GLY

4

ASN

ILE

HIS

LYS

PRO

GLY

GLN

5

VAL

GLU

VAL

LYS

SER

GLU

LYS

LEU

ASP

PHE

6

LYS

ASP

ARG

VAL

GLN

SER

LYS

ILE

GLY

SER

7

LEU

ASP

ASN

ILE

THR

HIS

VAL

PRO

GLY

8

GLY

ASN

LYS

ILE

GLU

THR

HIS

LYS

LEU

9

THR

PHE

ARG

GLU

ASN

ALA

LYS

ALA

LYS

THR

10

ASP

HIS

GLY

ALA

GLU

Samples:

sample_1: tau(297-391), [U-100% 13C; U-100% 15N], 300 uM; H2O 100%; sodium phosphate 10 mM; DTT 10 mM

sample_2: tau(297-391), [U-100% 13C; U-100% 15N], 300 uM; H2O 100%; sodium phosphate 10 mM

sample_conditions_1: ionic strength: 0 M; pH: 7.0; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 13C-13C DREAM	sample_1	isotropic	sample_conditions_1
2D 15N-13C NCA	sample_1	isotropic	sample_conditions_1
1D 13C RINEPT	sample_1	isotropic	sample_conditions_1
1D 13C CP	sample_1	isotropic	sample_conditions_1
2D 1H-13C INEPT HETCOR	sample_1	isotropic	sample_conditions_1
2D 15N-13C NCO	sample_1	isotropic	sample_conditions_1
3D NCOCA	sample_1	isotropic	sample_conditions_1
3D NCACB	sample_1	isotropic	sample_conditions_1
3D NCACO	sample_1	isotropic	sample_conditions_1
3D CANcoCA	sample_1	isotropic	sample_conditions_1
2D 13C-13C PARIS	sample_1	isotropic	sample_conditions_1
1D 13C CP	sample_2	isotropic	sample_conditions_1
1D 13C RINEPT	sample_2	isotropic	sample_conditions_1
2D 1H-13C INEPT HETCOR	sample_2	isotropic	sample_conditions_1

Software:

VNMRj v4.0 - collection

NMRPipe v9.4 - processing

CARA v1.9.1.7 - data analysis

NMR spectrometers:

Agilent DD2 600 MHz

UNP	P10636
AlphaFold	Q9UQ96