BMRB Entry 51483

Title:
Solid-state NMR assignment of fibrils formed by tau(297-391)
Deposition date:
2022-06-08
Original release date:
2023-01-30
Authors:
Siemer, Ansgar; Serpell, Louise
Citation:

Citation: Al-Hilaly, Youssra; Hurd, Connor; Rickard, Janet; Harrington, Charles; Storey, John; Wischik, Claude; Serpell, Louise; Siemer, Ansgar. "Solid-state NMR of paired helical filaments formed by the core tau fragment tau(297-391)"  Front. Neurosci. 16, 988074-988074 (2022).
PubMed: 36570831

Assembly members:

Assembly members:
entity_1, polymer, 95 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pRK172

Data sets:
Data typeCount
13C chemical shifts160
15N chemical shifts36

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1tau(297-391)1

Entities:

Entity 1, tau(297-391) 95 residues - Formula weight is not available

1   ILELYSHISVALPROGLYGLYGLYSERVAL
2   GLNILEVALTYRLYSPROVALASPLEUSER
3   LYSVALTHRSERLYSCYSGLYSERLEUGLY
4   ASNILEHISHISLYSPROGLYGLYGLYGLN
5   VALGLUVALLYSSERGLULYSLEUASPPHE
6   LYSASPARGVALGLNSERLYSILEGLYSER
7   LEUASPASNILETHRHISVALPROGLYGLY
8   GLYASNLYSLYSILEGLUTHRHISLYSLEU
9   THRPHEARGGLUASNALALYSALALYSTHR
10   ASPHISGLYALAGLU

Samples:

sample_1: tau(297-391), [U-100% 13C; U-100% 15N], 300 uM; H2O 100%; sodium phosphate 10 mM; DTT 10 mM

sample_2: tau(297-391), [U-100% 13C; U-100% 15N], 300 uM; H2O 100%; sodium phosphate 10 mM

sample_conditions_1: ionic strength: 0 M; pH: 7.0; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 13C-13C DREAMsample_1isotropicsample_conditions_1
2D 15N-13C NCAsample_1isotropicsample_conditions_1
1D 13C RINEPTsample_1isotropicsample_conditions_1
1D 13C CPsample_1isotropicsample_conditions_1
2D 1H-13C INEPT HETCORsample_1isotropicsample_conditions_1
2D 15N-13C NCOsample_1isotropicsample_conditions_1
3D NCOCAsample_1isotropicsample_conditions_1
3D NCACBsample_1isotropicsample_conditions_1
3D NCACOsample_1isotropicsample_conditions_1
3D CANcoCAsample_1isotropicsample_conditions_1
2D 13C-13C PARISsample_1isotropicsample_conditions_1
1D 13C CPsample_2isotropicsample_conditions_1
1D 13C RINEPTsample_2isotropicsample_conditions_1
2D 1H-13C INEPT HETCORsample_2isotropicsample_conditions_1

Software:

VNMRj v4.0 - collection

NMRPipe v9.4 - processing

CARA v1.9.1.7 - data analysis

NMR spectrometers:

  • Agilent DD2 600 MHz

Related Database Links:

UNP P10636
AlphaFold Q9UQ96