BMRB Entry 51481

Name: Chemical Shift Assignments of CT-IC 216-260
Published: 2022-12-12

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51481

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical Shift Assignments of CT-IC 216-260

Deposition date:

2022-06-03

Original release date:

2022-12-12

Authors:

Loening, Nikolaus

Citation:

Citation: Jara, Kayla; Loening, Nikolaus; Reardon, Patrick; Yu, Zhen; Woonnimani, Prajna; Brooks, Coban; Vesely, Cat; Barbar, Elisar. "Multivalency, autoinhibition, and protein disorder in the regulation of interactions of dynein intermediate chain with dynactin and the nuclear distribution protein" Elife 11, e80217-e80217 (2022).
PubMed: 36416224

Assembly members:

Assembly members:
entity_1, polymer, 46 residues, Formula weight is not available

Natural source:

Natural source: Common Name: ascomycete fungi Taxonomy ID: 209285 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Thermochaetoides thermophila

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-24

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAEELDAVTKSVEFMDFIDR STKVIEKALDQEYDILTDYT LQVHDV

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	156
15N chemical shifts	46
1H chemical shifts	322

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CTIC 216-260	1

Entities:

Entity 1, CTIC 216-260 46 residues - Formula weight is not available

First residue (G) is a non-native artifact

1

GLY

ALA

GLU

LEU

ASP

ALA

VAL

THR

LYS

2

SER

VAL

GLU

PHE

MET

ASP

PHE

ILE

ASP

ARG

3

SER

THR

LYS

VAL

ILE

GLU

LYS

ALA

LEU

ASP

4

GLN

GLU

TYR

ASP

ILE

LEU

THR

ASP

TYR

THR

5

LEU

GLN

VAL

HIS

ASP

VAL

Samples:

sample_1: CT-IC 216-260, [U-99% 15N], 550 uM; DSS 200 uM; sodium chloride 20 mM; sodium phosphate 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7.4; pressure: 1 atm; temperature: 283.1 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
3D 15N-separated NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v4.1.4 - collection, processing

CcpNMR v2.5.2 - chemical shift assignment

NMR spectrometers:

Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks