BMRB Entry 51479

Title:
Androgen Receptor AF1star
Deposition date:
2022-06-02
Original release date:
2022-09-30
Authors:
Garcia, Jesus; Fenwick, Robert; de Mol, Eva; Salvatella, Xavier
Citation:

Citation: Basu, Shaon; Martinez-Cristobal, Paula; Frigole-Vivas, Marta; Pesarrodona, Mireia; Lewis, Michael; Szulc, Elzbieta; Banuelos, C Adriana; Sanchez-Zarzalejo, Carolina; Bielskute, Stase; Zhu, Jiaqi; Pombo-Garcia, Karina; Garcia-Cabau, Carla; Zodi, Levente; Dockx, Hannes; Smak, Jordann; Kaur, Harpreet; Batlle, Cristina; Mateos, Borja; Biesaga, Mateusz; Escobedo, Albert; Bardia, Lidia; Verdaguer, Xavier; Ruffoni, Alessandro; Mawji, Nasrin; Wang, Jun; Obst, Jon; Tam, Teresa; Brun-Heath, Isabelle; Ventura, Salvador; Meierhofer, David; Garcia, Jesus; Robustelli, Paul; Stracker, Travis; Sadar, Marianne; Riera, Antoni; Hnisz, Denes; Salvatella, Xavier. "Rational optimization of a transcription factor activation domain inhibitor"  Nat. Struct. Mol. Biol. 30, 1958-1969 (2023).
PubMed: 38049566

Assembly members:

Assembly members:
entity_1, polymer, 308 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pDEST

Data sets:
Data typeCount
13C chemical shifts855
15N chemical shifts274
1H chemical shifts274

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Androgen Receptor AF1star1

Entities:

Entity 1, Androgen Receptor AF1star 308 residues - Formula weight is not available

1   GLYGLYLEUPROGLNGLNLEUPROALAPRO
2   PROASPGLUASPASPSERALAALAPROSER
3   THRLEUSERLEULEUGLYPROTHRPHEPRO
4   GLYLEUSERSERCYSSERALAASPLEULYS
5   ASPILELEUSERGLUALASERTHRMETGLN
6   LEULEUGLNGLNGLNGLNGLNGLUALAVAL
7   SERGLUGLYSERSERSERGLYARGALAARG
8   GLUALASERGLYALAPROTHRSERSERLYS
9   ASPASNTYRLEUGLYGLYTHRSERTHRILE
10   SERASPASNALALYSGLULEUCYSLYSALA
11   VALSERVALSERMETGLYLEUGLYVALGLU
12   ALALEUGLUHISLEUSERPROGLYGLUGLN
13   LEUARGGLYASPCYSMETTYRALAPROLEU
14   LEUGLYVALPROPROALAVALARGPROTHR
15   PROCYSALAPROLEUALAGLUCYSLYSGLY
16   SERLEULEUASPASPSERALAGLYLYSSER
17   THRGLUASPTHRALAGLUTYRSERPROPHE
18   LYSGLYGLYTYRTHRLYSGLYLEUGLUGLY
19   GLUSERLEUGLYCYSSERGLYSERALAALA
20   ALAGLYSERSERGLYTHRLEUGLULEUPRO
21   SERTHRLEUSERLEUTYRLYSSERGLYALA
22   LEUASPGLUALAALAALATYRGLNSERARG
23   ASPTYRTYRASNPHEPROLEUALALEUALA
24   GLYPROPROPROPROPROPROPROPROHIS
25   PROHISALAARGILELYSLEUGLUASNPRO
26   LEUASPTYRGLYSERALATRPALAALAALA
27   ALAALAGLNCYSARGTYRGLYASPLEUALA
28   SERLEUHISGLYALAGLYALAALAGLYPRO
29   GLYSERGLYSERPROSERALAALAALASER
30   SERSERTRPHISTHRLEUPHETHRALAGLU
31   GLUGLYGLNLEUTYRGLYPROCYS

Samples:

sample_1: Androgen Receptor AF1star, [U-100% 13C; U-100% 15N], 380 uM; D2O 10%; sodium phosphate 20 mM; sodium azide 0.05%; TCEP 1 mM

sample_conditions_1: ionic strength: 0 M; pH: 7.4; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCANHsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

NMRPipe - processing

CcpNMR - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks