BMRB Entry 51478

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51478

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Title:
Relaxation peaklist data of TcART
Deposition date:
2022-05-30
Original release date:
2022-06-22
Authors:
Lindemann, Florian; Schmieder, Peter; Oschkinat, Hartmut
Citation:

Citation: Belyy, Alexander; Lindemann, Florian; Roderer, Daniel; Funk, Johanna; Bardiaux, Benjamin; Protze, Jonas; Bieling, Peter; Oschkinat, Hartmut; Raunser, Stefan. "Mechanism of threonine ADP-ribosylation of F-actin by a Tc toxin"  Nat. Commun. 13, 4202-4202 (2022).
PubMed: 35858890

Assembly members:

Assembly members:
TcART, polymer, 194 residues, 21703.8062 Da.

Natural source:

Natural source:   Common Name: Photorhabdus luminescens   Taxonomy ID: 29488   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Photorhabdus luminescens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pB137

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TcART: MSTTSTNLQKKSFTLYRADN RSFEEMQSKFPEGFKAWTPL DTKMARQFASIFIGQKDTSN LPKETVKNISTWGAKPKLKD LSNYIKYTKDKSTVWVSTAI NTEAGGQSSGAPLHKIDMDL YEFAIDGQKLNPLPEGRTKN MVPSLLLDTPQIETSSIIAL NHGPVNDAEISFLTTIPLKN VKPHKRGTLEVLFQ

Data typeCount
15N chemical shifts174
1H chemical shifts174
T1 relaxation values118
T2 relaxation values116

Time Domain Data

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1TccC31

Entities:

Entity 1, TccC3 194 residues - 21703.8062 Da.

1   METSERTHRTHRSERTHRASNLEUGLNLYS
2   LYSSERPHETHRLEUTYRARGALAASPASN
3   ARGSERPHEGLUGLUMETGLNSERLYSPHE
4   PROGLUGLYPHELYSALATRPTHRPROLEU
5   ASPTHRLYSMETALAARGGLNPHEALASER
6   ILEPHEILEGLYGLNLYSASPTHRSERASN
7   LEUPROLYSGLUTHRVALLYSASNILESER
8   THRTRPGLYALALYSPROLYSLEULYSASP
9   LEUSERASNTYRILELYSTYRTHRLYSASP
10   LYSSERTHRVALTRPVALSERTHRALAILE
11   ASNTHRGLUALAGLYGLYGLNSERSERGLY
12   ALAPROLEUHISLYSILEASPMETASPLEU
13   TYRGLUPHEALAILEASPGLYGLNLYSLEU
14   ASNPROLEUPROGLUGLYARGTHRLYSASN
15   METVALPROSERLEULEULEUASPTHRPRO
16   GLNILEGLUTHRSERSERILEILEALALEU
17   ASNHISGLYPROVALASNASPALAGLUILE
18   SERPHELEUTHRTHRILEPROLEULYSASN
19   VALLYSPROHISLYSARGGLYTHRLEUGLU
20   VALLEUPHEGLN

Samples:

sample_1: TcART, [U-13C; U-15N], 500 uM; HEPES 20 mM; sodium chloride 150 mM

sample_conditions_1: delay time: 10.000 ms

sample_conditions_2: delay time: 900.000 ms

sample_conditions_3: delay time: 50.000 ms

sample_conditions_4: delay time: 600.000 ms

sample_conditions_5: delay time: 100.000 ms

sample_conditions_6: delay time: 400.000 ms

sample_conditions_7: delay time: 200.000 ms

sample_conditions_8: delay time: 1500.000 ms

sample_conditions_9: delay time: 15.000 ms

sample_conditions_10: delay time: 170.000 ms

sample_conditions_11: delay time: 35.000 ms

sample_conditions_12: delay time: 130.000 ms

sample_conditions_13: delay time: 40.000 ms

sample_conditions_14: delay time: 80.000 ms

sample_conditions_15: delay time: 60.000 ms

sample_conditions_16: delay time: 220.000 ms

sample_conditions_19: ionic strength: 150 mM; pH: 7.8; pressure: 1 atm; temperature: 280 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC/HMQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQC/HMQCsample_1isotropicsample_conditions_2
2D 1H-15N HSQC/HMQCsample_1isotropicsample_conditions_3
2D 1H-15N HSQC/HMQCsample_1isotropicsample_conditions_4
2D 1H-15N HSQC/HMQCsample_1isotropicsample_conditions_5
2D 1H-15N HSQC/HMQCsample_1isotropicsample_conditions_6
2D 1H-15N HSQC/HMQCsample_1isotropicsample_conditions_7
2D 1H-15N HSQC/HMQCsample_1isotropicsample_conditions_8
2D 1H-15N HSQC/HMQCsample_1isotropicsample_conditions_9
2D 1H-15N HSQC/HMQCsample_1isotropicsample_conditions_15
2D 1H-15N HSQC/HMQCsample_1isotropicsample_conditions_10
2D 1H-15N HSQC/HMQCsample_1isotropicsample_conditions_14
2D 1H-15N HSQC/HMQCsample_1isotropicsample_conditions_11
2D 1H-15N HSQC/HMQCsample_1isotropicsample_conditions_13
2D 1H-15N HSQC/HMQCsample_1isotropicsample_conditions_12
2D 1H-15N HSQC/HMQCsample_1isotropicsample_conditions_16

Software:

CcpNmr Analysis v2.4 - data analysis, peak picking

NMR spectrometers:

  • Bruker AVANCE III 600 MHz

Related Database Links:

UniProt Q8GF97
AlphaFold Q8GF97

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks