BMRB Entry 51474

Title:
tirE Coiled Coil   PubMed: 35889366
Deposition date:
2022-05-24
Original release date:
2022-08-08
Authors:
Lee, Eunjeong
Citation:

Citation: Lee, Eunjeong; Redzic, Jasmina; Nemkov, Travis; Saviola, Anthony; Dzieciatkowska, Monika; Hansen, Kirk; D'Alessandro, Angelo; Dinarello, Charles; Eisenmesser, Elan. "Human and Bacterial Toll-Interleukin Receptor Domains Exhibit Distinct Dynamic Features and Functions"  Molecules 27, 4494-4494 (2022).

Assembly members:

Assembly members:
entity_1, polymer, 146 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Enterococcus faecalis   Taxonomy ID: 1351   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Enterococcus faecalis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet21b

Experimental source:

Natural source:   Common Name: Enterococcus faecalis   Taxonomy ID: 1351   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Enterococcus faecalis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet21b

Data sets:
Data typeCount
13C chemical shifts328
15N chemical shifts106
1H chemical shifts106

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1tirE cc1

Entities:

Entity 1, tirE cc 146 residues - Formula weight is not available

1   METSERILEASNSERLEUGLNGLNLYSGLU
2   LYSSERLEULEUSERASPILETHRLYSLEU
3   GLUGLYASPTYRALAARGGLUGLNLYSLYS
4   ILEALAASNSERGLULYSLYSILEALAASP
5   SERSERLYSLYSILEGLUSERSERLYSSER
6   ILESERTHRILEARGSERGLNSERARGILE
7   LYSGLUSERGLUTHRLYSLYSSERLEUALA
8   SERLYSGLULYSSERALATHRILESERSER
9   LYSLEUALALYSLYSARGLYSGLULEUGLY
10   ASPILEGLNVALLYSLEUSERLYSGLNARG
11   THRILEGLUASNTHRGLNPHEGLNLYSASN
12   LEULYSLYSTHRTYRASPTHRGLNILEMET
13   LYSILELYSGLUTHRGLNTHRALAALALEU
14   GLNASNVALGLNSERGLULEUPROASNASP
15   PROSERLEUVALASNLYS

Samples:

sample_1: tirE CC, [U-13C; U-15N], 600 uM; D2O, [U-100% 2H], 8%; sodium chloride 150 mM; sodium phosphate 50 mM

sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1

Software:

CcpNMR - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks