BMRB Entry 51445

Name: Chemical shift assignments for alanine and valine methyls of Robo1-Ig1-2-LnLoop
Published: 2022-06-14

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51445

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Chemical shift assignments for alanine and valine methyls of Robo1-Ig1-2-LnLoop

Deposition date:

2022-05-16

Original release date:

2022-06-14

Authors:

Williams, Robert; Huang, Chin; Moremen, Kelley; Amster, I.; Prestegard, James

Citation:

Citation: Williams, Robert; Huang, Chin; Moremen, Kelley; Amster, I.; Prestegard, James. "NMR analysis suggests the terminal domains of Robo1 remain extended but are rigidified in the presence of heparan sulfate" Sci. Rep. 12, 14769-14769 (2022).
PubMed: 36042257

Assembly members:

Assembly members:
entity_1, polymer, 222 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: mammalian HEK Vector: pGEn2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSRLRQEDFPPRIVEHPSDL IVSKGEPATLNCKAEGRPTP TIEWYKGGERVETDKDDPRS HRMLLPSGSLFFLRIVHGRK SRPDEGVYVCVARNYLGEAV SHNASLEVAILRDDFRQNPS DVMVAVGEPAVMECQPPRGH PEPTISWKKDGSPLDDKDER ITIRCADTNNDGAYEGDELC KLMITYTRKSDAGKYVCVGT NMVGERESEVAELTVLERPS FV

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	29
1H chemical shifts	87

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Robo1-Ig1-2-LnLoop	1

Entities:

Entity 1, Robo1-Ig1-2-LnLoop 222 residues - Formula weight is not available

Numbering is consistent with uniprot Q9YN67 from 58-221. Then a 16 residue lanthanide binding tag replaces residues 222 and 223, resulting in the following sequence numbers differing from Q9YN67 by +14.

1

GLY

SER

ARG

LEU

ARG

GLN

GLU

ASP

PHE

PRO

2

PRO

ARG

ILE

VAL

GLU

HIS

PRO

SER

ASP

LEU

3

ILE

VAL

SER

LYS

GLY

GLU

PRO

ALA

THR

LEU

4

ASN

CYS

LYS

ALA

GLU

GLY

ARG

PRO

THR

PRO

5

THR

ILE

GLU

TRP

TYR

LYS

GLY

GLU

ARG

6

VAL

GLU

THR

ASP

LYS

ASP

PRO

ARG

SER

7

HIS

ARG

MET

LEU

PRO

SER

GLY

SER

LEU

8

PHE

LEU

ARG

ILE

VAL

HIS

GLY

ARG

LYS

9

SER

ARG

PRO

ASP

GLU

GLY

VAL

TYR

VAL

CYS

10

VAL

ALA

ARG

ASN

TYR

LEU

GLY

GLU

ALA

VAL

11

SER

HIS

ASN

ALA

SER

LEU

GLU

VAL

ALA

ILE

12

LEU

ARG

ASP

PHE

ARG

GLN

ASN

PRO

SER

13

ASP

VAL

MET

VAL

ALA

VAL

GLY

GLU

PRO

ALA

14

VAL

MET

GLU

CYS

GLN

PRO

ARG

GLY

HIS

15

PRO

GLU

PRO

THR

ILE

SER

TRP

LYS

ASP

16

GLY

SER

PRO

LEU

ASP

LYS

ASP

GLU

ARG

17

ILE

THR

ILE

ARG

CYS

ALA

ASP

THR

ASN

18

ASP

GLY

ALA

TYR

GLU

GLY

ASP

GLU

LEU

CYS

19

LYS

LEU

MET

ILE

THR

TYR

THR

ARG

LYS

SER

20

ASP

ALA

GLY

LYS

TYR

VAL

CYS

VAL

GLY

THR

21

ASN

MET

VAL

GLY

GLU

ARG

GLU

SER

GLU

VAL

22

ALA

GLU

LEU

THR

VAL

LEU

GLU

ARG

PRO

SER

23

PHE

VAL

Samples:

sample_1: Robo1-Ig1-2-LnLoop, [U-13C; 49% HB-Ala; 99% HG-Val], 300 uM; D2O, [U-99% 2H], 10%; TRIS 25 mM; NaCl 100 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 13C-detected HSQC	sample_1	isotropic	sample_conditions_1
3D 13C-detected NOESY	sample_1	isotropic	sample_conditions_1
2D 13C-detected TOCSY	sample_1	isotropic	sample_conditions_1

Software:

ASSIGN_SLPGUI v2.4.6 - chemical shift assignment

NMR spectrometers:

Bruker AVANCE NEO 900 MHz