BMRB Entry 51440

Title:
Identification and characterization of an RRM-containing, RNA binding protein in Acinetobacter Baumannii
Deposition date:
2022-05-13
Original release date:
2022-09-19
Authors:
Ciani, Caterina; Perez-Rafols, Anna; Bonomo, Isabelle; Micaelli, Mariachiara; Esposito, Alfonso; Zucal, Chiara; Belli, Romina; D'Agostino, Vito Giuseppe; Bianconi, Irene; Calderone, Vito; Cerofolini, Linda; Fragai, Marco; Provenzani, Alessandro
Citation:

Citation: Ciani, Caterina; Perez-Rafols, Anna; Bonomo, Isabelle; Micaelli, Mariachiara; Esposito, Alfonso; Zucal, Chiara; Belli, Romina; D'Agostino, Vito Giuseppe; Bianconi, Irene; Calderone, Vito; Cerofolini, Linda; Massidda, Orietta; Whalen, Michael Bernard; Fragai, Marco; Provenzani, Alessandro. "Identification and characterization of an RRM-containing, RNA binding protein in Acinetobacter Baumannii"  Biomolecules 12, 922-922 (2022).
PubMed: 35883478

Assembly members:

Assembly members:
entity_1, polymer, 107 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Acinetobacter Baumannii   Taxonomy ID: 470   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Acinetobacter Baumannii

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-30a(+)

Data sets:
Data typeCount
13C chemical shifts232
15N chemical shifts78
1H chemical shifts78

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AB-Elavl1

Entities:

Entity 1, AB-Elavl 107 residues - Formula weight is not available

1   METHISMETILELEULYSCYSILELEUALA
2   PHELEULEUMETVALASNGLUGLYTRPLYS
3   METLYSILELEUVALARGASNLEUASPARG
4   SERVALTHRGLUALAGLUVALLEUGLULEU
5   PHELYSALATYRGLYLYSVALGLUSERCYS
6   VALVALVALTHRASPLYSASPTHRGLYLYS
7   SERLYSGLYPHEGLYPHEVALGLUMETPRO
8   ASNPROARGGLUALAILELYSALAILELYS
9   GLYLEUASNTHRLEULYSVALLYSGLYTYR
10   GLYILEARGVALLYSALAALAGLUGLULEU
11   GLUHISHISHISHISHISHIS

Samples:

sample_1: AB-Elavl, [U-100% 13C; U-100% 15N], 300 uM; HEPES 20 mM; NaCl 150 mM; MgCl2 3 mM; AEBSF protease inhibitor 1 mM

sample_conditions_1: ionic strength: 0.309 M; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

TOPSPIN v4.1.3 - collection, processing

CARA v1.8 - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE NEO 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks