BMRB Entry 51439

Name: 1H, 13C,15N assignment of human CISD3 mitochondrial protein
Published: 2022-12-12

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51439

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C,15N assignment of human CISD3 mitochondrial protein

Deposition date:

2022-05-13

Original release date:

2022-12-12

Authors:

Silva, Jose; Grifagni, Deborah; Cantini, Francesca; Piccioli, Mario; Banci, Lucia

Citation:

Citation: Grifagni, Deborah; Silva, Jose; Cantini, Francesca; Piccioli, Mario; Banci, Lucia. "Relaxation-based NMR assignment: Spotlights on ligand binding sites in human CISD3" J. Inorg. Biochem. 239, 112089-112089 (2022).
PubMed: 36502664

Assembly members:

Assembly members:
entity_1, polymer, 93 residues, 10360.14 Da.
entity_FES, non-polymer, 175.820 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PET28a(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGARSVVALKTPIKVELVAG KTYRWCVCGRSKKQPFCDGS HFFQRTGLSPLKFKAQETRM VALCTCKATQRPPYCDGTHR SERVQKAEVGSPL

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	166
15N chemical shifts	60
1H chemical shifts	59

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CISD3	1
2	Fe2-S2, 1	2
3	Fe2-S2, 2	2

Entities:

Entity 1, CISD3 93 residues - 10360.14 Da.

Construct of CISD3

1

MET

GLY

ALA

ARG

SER

VAL

ALA

LEU

LYS

2

THR

PRO

ILE

LYS

VAL

GLU

LEU

VAL

ALA

GLY

3

LYS

THR

TYR

ARG

TRP

CYS

VAL

CYS

GLY

ARG

4

SER

LYS

GLN

PRO

PHE

CYS

ASP

GLY

SER

5

HIS

PHE

GLN

ARG

THR

GLY

LEU

SER

PRO

6

LEU

LYS

PHE

LYS

ALA

GLN

GLU

THR

ARG

MET

7

VAL

ALA

LEU

CYS

THR

CYS

LYS

ALA

THR

GLN

8

ARG

PRO

TYR

CYS

ASP

GLY

THR

HIS

ARG

9

SER

GLU

ARG

VAL

GLN

LYS

ALA

GLU

VAL

GLY

10

SER

PRO

LEU

Entity 2, Fe2-S2, 1 - Fe2 S2 - 175.820 Da.

1

FES

Samples:

sample_1: CISD3, [U-99% 13C; U-99% 15N], 0.5 mM; sodium phosphate 50 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D CBCACONH	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC ANTIPHASE	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC ANTIPHASE INVERSION RECOVERY	sample_1	isotropic	sample_conditions_1
2D 13C-detected CACO	sample_1	isotropic	sample_conditions_1
2D 13C-detected CON	sample_1	isotropic	sample_conditions_1
2D 13C-detected CON PARAMAGNETIC	sample_1	isotropic	sample_conditions_1

Software:

CARA v1.9 - chemical shift assignment

TOPSPIN v3.6 - collection

NMR spectrometers:

Bruker AVANCE NEO 700 MHz
Bruker AVANCE NEO 500 MHz