BMRB Entry 51437

Name: Backbone assignment of S. pombe PCNA
Published: 2024-01-12

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51437

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignment of S. pombe PCNA

Deposition date:

2022-05-12

Original release date:

2024-01-12

Authors:

Prestel, Andreas; Kragelund, Birthe

Citation:

Citation: Olsen, Johan; Prestel, Andreas; Kassem, Noah; Broendum, Sebastian; Shamim, Hossain Mohammad; Simonsen, Signe; Grysbak, Martin; Mortensen, Josefine; Rytkjar, Louise Lund; Haxholm, Gitte; Marabini, Riccardo; Holmberg, Christian; Carr, Antony; Crehuet, Ramon; Nielsen, Olaf; Kragelund, Birthe. "Checkpoint activation by Spd1: a competition-based system relying on tandem disordered PCNA binding motifs" Nucleic Acids Res. 52, 2030-2044 (2024).
PubMed: 38261971

Assembly members:

Assembly members:
entity_1, polymer, 260 residues, Formula weight is not available

Natural source:

Natural source: Common Name: fission yeast Taxonomy ID: 4896 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Schizosaccharomyces pombe

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET24a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MLEARFQQAALLKKLLDAIK ELVTDANFDCNDNGISLQAM DSSHVALVSMLIKSDGFEPY RCDRNIALGINLNALSKVLR CAQNEDLVTLKAEDTPEVLN LVFESEKNDRISDYDVKLMD IDQEHLGIPDIEYDATITMP AAEFQRITRDLLTLSDSVTI NASKEGVRFSCKGDIGNGST TLKQHTDLSDQDQSIEISLT QAVTLTFSLKYLAQFTKATP LATRVTLSMSNDVPLLVEYK MESGFLRFYLAPKIGEEDEE

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	684
15N chemical shifts	239
1H chemical shifts	239

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PCNA	1

Entities:

Entity 1, PCNA 260 residues - Formula weight is not available

1	MET	LEU	GLU	ALA	ARG	PHE	GLN	GLN	ALA	ALA
2	LEU	LEU	LYS	LYS	LEU	LEU	ASP	ALA	ILE	LYS
3	GLU	LEU	VAL	THR	ASP	ALA	ASN	PHE	ASP	CYS
4	ASN	ASP	ASN	GLY	ILE	SER	LEU	GLN	ALA	MET
5	ASP	SER	SER	HIS	VAL	ALA	LEU	VAL	SER	MET
6	LEU	ILE	LYS	SER	ASP	GLY	PHE	GLU	PRO	TYR
7	ARG	CYS	ASP	ARG	ASN	ILE	ALA	LEU	GLY	ILE
8	ASN	LEU	ASN	ALA	LEU	SER	LYS	VAL	LEU	ARG
9	CYS	ALA	GLN	ASN	GLU	ASP	LEU	VAL	THR	LEU
10	LYS	ALA	GLU	ASP	THR	PRO	GLU	VAL	LEU	ASN
11	LEU	VAL	PHE	GLU	SER	GLU	LYS	ASN	ASP	ARG
12	ILE	SER	ASP	TYR	ASP	VAL	LYS	LEU	MET	ASP
13	ILE	ASP	GLN	GLU	HIS	LEU	GLY	ILE	PRO	ASP
14	ILE	GLU	TYR	ASP	ALA	THR	ILE	THR	MET	PRO
15	ALA	ALA	GLU	PHE	GLN	ARG	ILE	THR	ARG	ASP
16	LEU	LEU	THR	LEU	SER	ASP	SER	VAL	THR	ILE
17	ASN	ALA	SER	LYS	GLU	GLY	VAL	ARG	PHE	SER
18	CYS	LYS	GLY	ASP	ILE	GLY	ASN	GLY	SER	THR
19	THR	LEU	LYS	GLN	HIS	THR	ASP	LEU	SER	ASP
20	GLN	ASP	GLN	SER	ILE	GLU	ILE	SER	LEU	THR
21	GLN	ALA	VAL	THR	LEU	THR	PHE	SER	LEU	LYS
22	TYR	LEU	ALA	GLN	PHE	THR	LYS	ALA	THR	PRO
23	LEU	ALA	THR	ARG	VAL	THR	LEU	SER	MET	SER
24	ASN	ASP	VAL	PRO	LEU	LEU	VAL	GLU	TYR	LYS
25	MET	GLU	SER	GLY	PHE	LEU	ARG	PHE	TYR	LEU
26	ALA	PRO	LYS	ILE	GLY	GLU	GLU	ASP	GLU	GLU

Samples:

sample_1: PCNA, [U-13C; U-15N; U-2H], 1 ± 0.05 mM; DSS 125 ± 5 uM; sodium chloride 100 ± 5 mM; DTT 2 ± 0.1 mM; sodium phosphate 10 ± 0.1 mM

sample_conditions_1: ionic strength: 120 mM; pH: 7; pressure: 1 atm; temperature: 308.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N TROSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1

Software:

qMDD v3.2 - processing

ANALYSIS v2.5 - data analysis

TOPSPIN v3.5 - collection

NMR spectrometers:

Bruker AVANCE III 750 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

1	MET	LEU	GLU	ALA	ARG	PHE	GLN	GLN	ALA	ALA
2	LEU	LEU	LYS	LYS	LEU	LEU	ASP	ALA	ILE	LYS
3	GLU	LEU	VAL	THR	ASP	ALA	ASN	PHE	ASP	CYS
4	ASN	ASP	ASN	GLY	ILE	SER	LEU	GLN	ALA	MET
5	ASP	SER	SER	HIS	VAL	ALA	LEU	VAL	SER	MET
6	LEU	ILE	LYS	SER	ASP	GLY	PHE	GLU	PRO	TYR
7	ARG	CYS	ASP	ARG	ASN	ILE	ALA	LEU	GLY	ILE
8	ASN	LEU	ASN	ALA	LEU	SER	LYS	VAL	LEU	ARG
9	CYS	ALA	GLN	ASN	GLU	ASP	LEU	VAL	THR	LEU
10	LYS	ALA	GLU	ASP	THR	PRO	GLU	VAL	LEU	ASN
11	LEU	VAL	PHE	GLU	SER	GLU	LYS	ASN	ASP	ARG
12	ILE	SER	ASP	TYR	ASP	VAL	LYS	LEU	MET	ASP
13	ILE	ASP	GLN	GLU	HIS	LEU	GLY	ILE	PRO	ASP
14	ILE	GLU	TYR	ASP	ALA	THR	ILE	THR	MET	PRO
15	ALA	ALA	GLU	PHE	GLN	ARG	ILE	THR	ARG	ASP
16	LEU	LEU	THR	LEU	SER	ASP	SER	VAL	THR	ILE
17	ASN	ALA	SER	LYS	GLU	GLY	VAL	ARG	PHE	SER
18	CYS	LYS	GLY	ASP	ILE	GLY	ASN	GLY	SER	THR
19	THR	LEU	LYS	GLN	HIS	THR	ASP	LEU	SER	ASP
20	GLN	ASP	GLN	SER	ILE	GLU	ILE	SER	LEU	THR
21	GLN	ALA	VAL	THR	LEU	THR	PHE	SER	LEU	LYS
22	TYR	LEU	ALA	GLN	PHE	THR	LYS	ALA	THR	PRO
23	LEU	ALA	THR	ARG	VAL	THR	LEU	SER	MET	SER
24	ASN	ASP	VAL	PRO	LEU	LEU	VAL	GLU	TYR	LYS
25	MET	GLU	SER	GLY	PHE	LEU	ARG	PHE	TYR	LEU
26	ALA	PRO	LYS	ILE	GLY	GLU	GLU	ASP	GLU	GLU

1	MET	LEU	GLU	ALA	ARG	PHE	GLN	GLN	ALA	ALA
2	LEU	LEU	LYS	LYS	LEU	LEU	ASP	ALA	ILE	LYS
3	GLU	LEU	VAL	THR	ASP	ALA	ASN	PHE	ASP	CYS
4	ASN	ASP	ASN	GLY	ILE	SER	LEU	GLN	ALA	MET
5	ASP	SER	SER	HIS	VAL	ALA	LEU	VAL	SER	MET
6	LEU	ILE	LYS	SER	ASP	GLY	PHE	GLU	PRO	TYR
7	ARG	CYS	ASP	ARG	ASN	ILE	ALA	LEU	GLY	ILE
8	ASN	LEU	ASN	ALA	LEU	SER	LYS	VAL	LEU	ARG
9	CYS	ALA	GLN	ASN	GLU	ASP	LEU	VAL	THR	LEU
10	LYS	ALA	GLU	ASP	THR	PRO	GLU	VAL	LEU	ASN
11	LEU	VAL	PHE	GLU	SER	GLU	LYS	ASN	ASP	ARG
12	ILE	SER	ASP	TYR	ASP	VAL	LYS	LEU	MET	ASP
13	ILE	ASP	GLN	GLU	HIS	LEU	GLY	ILE	PRO	ASP
14	ILE	GLU	TYR	ASP	ALA	THR	ILE	THR	MET	PRO
15	ALA	ALA	GLU	PHE	GLN	ARG	ILE	THR	ARG	ASP
16	LEU	LEU	THR	LEU	SER	ASP	SER	VAL	THR	ILE
17	ASN	ALA	SER	LYS	GLU	GLY	VAL	ARG	PHE	SER
18	CYS	LYS	GLY	ASP	ILE	GLY	ASN	GLY	SER	THR
19	THR	LEU	LYS	GLN	HIS	THR	ASP	LEU	SER	ASP
20	GLN	ASP	GLN	SER	ILE	GLU	ILE	SER	LEU	THR
21	GLN	ALA	VAL	THR	LEU	THR	PHE	SER	LEU	LYS
22	TYR	LEU	ALA	GLN	PHE	THR	LYS	ALA	THR	PRO
23	LEU	ALA	THR	ARG	VAL	THR	LEU	SER	MET	SER
24	ASN	ASP	VAL	PRO	LEU	LEU	VAL	GLU	TYR	LYS
25	MET	GLU	SER	GLY	PHE	LEU	ARG	PHE	TYR	LEU
26	ALA	PRO	LYS	ILE	GLY	GLU	GLU	ASP	GLU	GLU

1	MET	LEU	GLU	ALA	ARG	PHE	GLN	GLN	ALA	ALA
2	LEU	LEU	LYS	LYS	LEU	LEU	ASP	ALA	ILE	LYS
3	GLU	LEU	VAL	THR	ASP	ALA	ASN	PHE	ASP	CYS
4	ASN	ASP	ASN	GLY	ILE	SER	LEU	GLN	ALA	MET
5	ASP	SER	SER	HIS	VAL	ALA	LEU	VAL	SER	MET
6	LEU	ILE	LYS	SER	ASP	GLY	PHE	GLU	PRO	TYR
7	ARG	CYS	ASP	ARG	ASN	ILE	ALA	LEU	GLY	ILE
8	ASN	LEU	ASN	ALA	LEU	SER	LYS	VAL	LEU	ARG
9	CYS	ALA	GLN	ASN	GLU	ASP	LEU	VAL	THR	LEU
10	LYS	ALA	GLU	ASP	THR	PRO	GLU	VAL	LEU	ASN
11	LEU	VAL	PHE	GLU	SER	GLU	LYS	ASN	ASP	ARG
12	ILE	SER	ASP	TYR	ASP	VAL	LYS	LEU	MET	ASP
13	ILE	ASP	GLN	GLU	HIS	LEU	GLY	ILE	PRO	ASP
14	ILE	GLU	TYR	ASP	ALA	THR	ILE	THR	MET	PRO
15	ALA	ALA	GLU	PHE	GLN	ARG	ILE	THR	ARG	ASP
16	LEU	LEU	THR	LEU	SER	ASP	SER	VAL	THR	ILE
17	ASN	ALA	SER	LYS	GLU	GLY	VAL	ARG	PHE	SER
18	CYS	LYS	GLY	ASP	ILE	GLY	ASN	GLY	SER	THR
19	THR	LEU	LYS	GLN	HIS	THR	ASP	LEU	SER	ASP
20	GLN	ASP	GLN	SER	ILE	GLU	ILE	SER	LEU	THR
21	GLN	ALA	VAL	THR	LEU	THR	PHE	SER	LEU	LYS
22	TYR	LEU	ALA	GLN	PHE	THR	LYS	ALA	THR	PRO
23	LEU	ALA	THR	ARG	VAL	THR	LEU	SER	MET	SER
24	ASN	ASP	VAL	PRO	LEU	LEU	VAL	GLU	TYR	LYS
25	MET	GLU	SER	GLY	PHE	LEU	ARG	PHE	TYR	LEU
26	ALA	PRO	LYS	ILE	GLY	GLU	GLU	ASP	GLU	GLU