BMRB Entry 51432

Name: Bcl-xl-delC UV exposed assigned chemical shifts used for TALOS calculation
Published: 2022-05-17

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51432

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Bcl-xl-delC UV exposed assigned chemical shifts used for TALOS calculation

Deposition date:

2022-05-06

Original release date:

2022-05-17

Authors:

Mishra, Pushpa

Citation:

Citation: Bera, Akash; Singh, Suraj; D'Souza, Jacinta; Hosur, Ramakrishna; Mishra, Pushpa. "Effect of UV Stress on the Structure and Function of Pro-apoptotic Bid and Anti-apoptotic Bcl-xl proteins" Chembiochem ., e202200682-e202200682 (2023).
PubMed: 36597005

Assembly members:

Assembly members:
entity_1, polymer, 171 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSQSNRELVVDFLSYKLSQK GYSWSQFSDVEENRTEAPEG TESEAVKQALREAGDEFELR YRRAFSDLTSQLHITPGTAY QSFEQVVNELFRDGVNWGRI VAFFSFGGALCVESVDKEMQ VLVSRIAAWMATYLNDHLEP WIQENGGWDTFVELYGNNAA AESRKGQERLE

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	42
15N chemical shifts	60
1H chemical shifts	60

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Bcl-xl	1

Entities:

Entity 1, Bcl-xl 171 residues - Formula weight is not available

1

MET

SER

GLN

SER

ASN

ARG

GLU

LEU

VAL

2

ASP

PHE

LEU

SER

TYR

LYS

LEU

SER

GLN

LYS

3

GLY

TYR

SER

TRP

SER

GLN

PHE

SER

ASP

VAL

4

GLU

ASN

ARG

THR

GLU

ALA

PRO

GLU

GLY

5

THR

GLU

SER

GLU

ALA

VAL

LYS

GLN

ALA

LEU

6

ARG

GLU

ALA

GLY

ASP

GLU

PHE

GLU

LEU

ARG

7

TYR

ARG

ALA

PHE

SER

ASP

LEU

THR

SER

8

GLN

LEU

HIS

ILE

THR

PRO

GLY

THR

ALA

TYR

9

GLN

SER

PHE

GLU

GLN

VAL

ASN

GLU

LEU

10

PHE

ARG

ASP

GLY

VAL

ASN

TRP

GLY

ARG

ILE

11

VAL

ALA

PHE

SER

PHE

GLY

ALA

LEU

12

CYS

VAL

GLU

SER

VAL

ASP

LYS

GLU

MET

GLN

13

VAL

LEU

VAL

SER

ARG

ILE

ALA

TRP

MET

14

ALA

THR

TYR

LEU

ASN

ASP

HIS

LEU

GLU

PRO

15

TRP

ILE

GLN

GLU

ASN

GLY

TRP

ASP

THR

16

PHE

VAL

GLU

LEU

TYR

GLY

ASN

ALA

17

ALA

GLU

SER

ARG

LYS

GLY

GLN

GLU

ARG

LEU

18

GLU

Samples:

sample_1: Bcl-xl, [U-99% 15N], 200 uM; Bcl-xl, [U-99% 13C; U-99% 15N], 400 uM; sodium phosphate 20 mM; DTT 2 mM; EDTA 1 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

Software:

SPARKY - data analysis

CARA - chemical shift assignment

TOPSPIN v3.6.3 - processing

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks