BMRB Entry 51422

Title:
1H, 13C and 15N backbone chemical shifts for human calmodulin in aqueous solution
Deposition date:
2022-04-28
Original release date:
2022-10-31
Authors:
Jimenez, M. Angeles; Comas, Laura; Pantoja-Uceda, David; Valdes, Patricia
Citation:

Citation: Labat-de-Hoz, Leticia; Comas, Laura; Rubio-Ramos, Armando; Casares-Arias, Javier; Fernandez-Martin, Laura; Pantoja-Uceda, David; Martin, M. Teresa; Kremer, Leonor; Jimenez, M. Angeles; Correas, Isabel; Alonso, Miguel. "Structure and function of the N-terminal extension of the formin INF2"  Cell. Mol. Life Sci. 79, 571-571 (2022).
PubMed: 36306014

Assembly members:

Assembly members:
entity_1, polymer, 148 residues, Formula weight is not available
entity_CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: R701-X65-527

Data sets:
Data typeCount
13C chemical shifts431
15N chemical shifts145
1H chemical shifts145

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CaM1
2calcium cation, 12
3calcium cation, 22
4calcium cation, 32
5calcium cation, 42

Entities:

Entity 1, CaM 148 residues - Formula weight is not available

1   ALAASPGLNLEUTHRGLUGLUGLNILEALA
2   GLUPHELYSGLUALAPHESERLEUPHEASP
3   LYSASPGLYASPGLYTHRILETHRTHRLYS
4   GLULEUGLYTHRVALMETARGSERLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   METILEASNGLUVALASPALAASPGLYASN
7   GLYTHRILEASPPHEPROGLUPHELEUTHR
8   METMETALAARGLYSMETLYSASPTHRASP
9   SERGLUGLUGLUILEARGGLUALAPHEARG
10   VALPHEASPLYSASPGLYASNGLYTYRILE
11   SERALAALAGLULEUARGHISVALMETTHR
12   ASNLEUGLYGLULYSLEUTHRASPGLUGLU
13   VALASPGLUMETILEARGGLUALAASPILE
14   ASPGLYASPGLYGLNVALASNTYRGLUGLU
15   PHEVALGLNMETMETTHRALALYS

Entity 2, calcium cation, 1 - Ca - 40.078 Da.

1   CA

Samples:

sample_1: CaM, [U-100% 13C; U-100% 15N], 0.25 mM; D2O, [U-2H], 10 % v/v; H2O 90 % v/v; Tris-HCl 20 mM; NaCl, natural abundance, 10 mM; CaCl2, natural abundance, 5 mM

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection, processing

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE NEO 800 MHz MHz

Related Database Links:

NCBI NP_001350599.1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks