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BMRB Entry 51420 BMRB - Biological Magnetic Resonance Bank

BMRB Entry 51420

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51420

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for EZH2 loop at 310K and 278K
Deposition date:
2022-04-28
Original release date:
2022-06-03
Authors:
Szabo, Csenge; Sebak, Fanni; Bodor, Andrea
Citation:

Citation: Szabo, Csenge; Szabo, Beata; Sebak, Fanni; Bermel, Wolfgang; Tantos, Agnes; Bodor, Andrea. "The Disordered EZH2 Loop: Atomic Level Characterization by 1HN- and 1Ha-Detected NMR Approaches, Interaction with the Long Noncoding HOTAIR RNA"  Int. J. Mol. Sci. 23, 6150-6150 (2022).

Assembly members:

Assembly members:
entity_1, polymer, 106 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22b (+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MDAERIKTPPKRPGGRRRGR LPNNSSRPSTPTINVLESKD TDSDREAGTETGGENNDKEE EEKKDETSSSSEANSRCQTP IKMKPNIEPPENVEWSGALE HHHHHH

Data sets:
Data typeCount
13C chemical shifts476
15N chemical shifts185
1H chemical shifts483

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1EZH2 loop1

Entities:

Entity 1, EZH2 loop 106 residues - Formula weight is not available

Residues 338-339 and 436-443 represent cloning artifacts.

1   METASPALAGLUARGILELYSTHRPROPRO
2   LYSARGPROGLYGLYARGARGARGGLYARG
3   LEUPROASNASNSERSERARGPROSERTHR
4   PROTHRILEASNVALLEUGLUSERLYSASP
5   THRASPSERASPARGGLUALAGLYTHRGLU
6   THRGLYGLYGLUASNASNASPLYSGLUGLU
7   GLUGLULYSLYSASPGLUTHRSERSERSER
8   SERGLUALAASNSERARGCYSGLNTHRPRO
9   ILELYSMETLYSPROASNILEGLUPROPRO
10   GLUASNVALGLUTRPSERGLYALALEUGLU
11   HISHISHISHISHISHIS

Samples:

sample_1: EZH2 loop, [U-100% 13C; U-100% 15N], 1 mM; PBS 150 mM; TCEP 10 mM; sodium azide 3 mM; DSS 1%

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 310 K

sample_conditions_2: pH: 7; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D SHACA-HSQCsample_1isotropicsample_conditions_1
3D HCANsample_1isotropicsample_conditions_1
3D HCA(CO)Nsample_1isotropicsample_conditions_1
3D HCA(N)COsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_2
3D HNCAsample_1isotropicsample_conditions_2
3D HN(CO)CAsample_1isotropicsample_conditions_2
3D HNCACBsample_1isotropicsample_conditions_2
3D HN(CO)CACBsample_1isotropicsample_conditions_2
3D HNCOsample_1isotropicsample_conditions_2
3D HNCACOsample_1isotropicsample_conditions_2
3D CCCONHsample_1isotropicsample_conditions_2
2D SHCA-HSQCsample_1isotropicsample_conditions_2
3D HCANsample_1isotropicsample_conditions_2
3D HCA(CO)Nsample_1isotropicsample_conditions_2
3D HCA(N)COsample_1isotropicsample_conditions_2

Software:

TOPSPIN - collection, processing

CARA - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks