BMRB Entry 51419

Name: Backbone chemical shift assignments of R86A mutant of the Akt1 PH domain
Published: 2022-10-11

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51419

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone chemical shift assignments of R86A mutant of the Akt1 PH domain

Deposition date:

2022-04-26

Original release date:

2022-10-11

Authors:

Viennet, Thibault; Bae, Hwan; Chu, Nam; Cole, Philip; Arthanari, Haribabu

Citation:

Citation: Bae, Hwan; Viennet, Thibault; Park, Eunyoung; Chu, Nam; Salguero, Antonieta; Eck, Michael; Arthanari, Haribabu; Cole, Philip. "PH domain-mediated autoinhibition and oncogenic activation of Akt" eLife 11, e80148-e80148 (2022).
PubMed: 35968932

Assembly members:

Assembly members:
entity_1, polymer, 121 residues, 14436 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pTXB1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSDVAIVKEGWLHKRGEYIK TWRPRYFLLKNDGTFIGYKE RPQDVDQREAPLNNFSVAQC QLMKTERPRPNTFIIRCLQW TTVIEATFHVETPEEREEWT TAIQTVADGLKKQEEEEMDF R

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	283
15N chemical shifts	94
1H chemical shifts	184

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Akt_PH_R86A	1

Entities:

Entity 1, Akt_PH_R86A 121 residues - 14436 Da.

1

MET

SER

ASP

VAL

ALA

ILE

VAL

LYS

GLU

GLY

2

TRP

LEU

HIS

LYS

ARG

GLY

GLU

TYR

ILE

LYS

3

THR

TRP

ARG

PRO

ARG

TYR

PHE

LEU

LYS

4

ASN

ASP

GLY

THR

PHE

ILE

GLY

TYR

LYS

GLU

5

ARG

PRO

GLN

ASP

VAL

ASP

GLN

ARG

GLU

ALA

6

PRO

LEU

ASN

PHE

SER

VAL

ALA

GLN

CYS

7

GLN

LEU

MET

LYS

THR

GLU

ARG

PRO

ARG

PRO

8

ASN

THR

PHE

ILE

ARG

CYS

LEU

GLN

TRP

9

THR

VAL

ILE

GLU

ALA

THR

PHE

HIS

VAL

10

GLU

THR

PRO

GLU

ARG

GLU

TRP

THR

11

THR

ALA

ILE

GLN

THR

VAL

ALA

ASP

GLY

LEU

12

LYS

GLN

GLU

MET

ASP

PHE

13

ARG

Samples:

sample_1: Akt_PH_R86A, [U-99% 13C; U-99% 15N], 600 uM; HEPES 25 mM; sodium chloride 500 mM; TCEP 1 mM; D2O, [U-2H], 5%

sample_conditions_1: ionic strength: 0.529 M; pH: 6.5; pressure: 1 atm; temperature: 286 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR v2.4.2 - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 750 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks