BMRB Entry 51410

Title:
Bicelle-bound conformation of HIV-1 gp41 ectodomain
Deposition date:
2022-04-23
Original release date:
2022-07-03
Authors:
Chiliveri, Sai Chaitanya; Bax, Ad
Citation:

Citation: Chiliveri, Sai Chaitanya; Louis, John; Best, Robert; Bax, Ad. "Real-time Exchange of the Lipid-bound Intermediate and Post-fusion States of the HIV-1 gp41 Ectodomain"  J. Mol. Biol. 434, 167683-167683 (2022).
PubMed: 35700771

Assembly members:

Assembly members:
entity_1, polymer, 72 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: HIV-1   Taxonomy ID: 11676   Superkingdom: Viruses   Kingdom: not available   Genus/species: Lentivirus HIV-1

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pJ414

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts135
15N chemical shifts68
1H chemical shifts68

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HIV-1 gp411

Entities:

Entity 1, HIV-1 gp41 72 residues - Formula weight is not available

-3,G. -2,S. -1,H. 0,M. 1,S. 2,G. 3,I. 4,V. 5,Q. 6,Q. 7,Q. 8,N. 9,N. 10,L. 11,L. 12,R. 13,A. 14,I. 15,E. 16,A. 17,Q. 18,Q. 19,H. 20,L. 21,A. 22,Q. 23,L. 24,T. 25,V. 26,W. 27,G. 28,I. 29,K. 30,Q. 31,L. 32,Q. 33,A. 34,R. 35,S. 36,G. 37,G. 38,R. 39,G. 40,G. 41,W. 42,M. 43,E. 44,W. 45,D. 46,R. 47,E. 48,I. 49,N. 50,N. 51,Y. 52,T. 53,S. 54,L. 55,I. 56,H. 57,S. 58,L. 59,I. 60,E. 61,E. 62,S. 63,Q. 64,N. 65,Q. 66,Q. 67,E. 68,K.

1   GLYSERHISMETSERGLYILEVALGLNGLN
2   GLNASNASNLEULEUARGALAILEGLUALA
3   GLNGLNHISLEUALAGLNLEUTHRVALTRP
4   GLYILELYSGLNLEUGLNALAARGSERGLY
5   GLYARGGLYGLYTRPMETGLUTRPASPARG
6   GLUILEASNASNTYRTHRSERLEUILEHIS
7   SERLEUILEGLUGLUSERGLNASNGLNGLN
8   GLULYS

Samples:

sample_1: HIV-1 gp41 ectodomain, [U-100% 13C; U-100% 15N], 200 uM; DMPC/DHPC 200 mM; sodium phosphate buffer (pH 6) 20 mM; sodium chloride 30 mM; imidazole 1 mM; ethylenediaminetetraacetic acid 0.5 mM; sodium trimethylsilylpropanesulfonate 100 uM

sample_2: HIV-1 gp41 ectodomain, [U-100% 15N; U-99% 2H], 200 uM; DMPC/DHPC 200 mM; sodium phosphate buffer (pH 6) 20 mM; sodium chloride 30 mM; imidazole 1 mM; ethylenediaminetetraacetic acid 0.5 mM; sodium trimethylsilylpropanesulfonate 100 uM

sample_conditions_1: ionic strength: 50 mM; pH: 6; pressure: 1 atm; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D 15N-separated NOESYsample_2isotropicsample_conditions_1

Software:

TOPSPIN v3 - collection

NMRPipe - processing

CcpNMR v3 - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 700 MHz

Related Database Links:

BMRB 19750

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks