BMRB Entry 51408

Title:
1H and 13C chemical shifts for a peptide encompassing residues 2-36 of the human formin INF2 in 30% TFE
Deposition date:
2022-04-21
Original release date:
2022-10-31
Authors:
Jimenez, M. Angeles
Citation:

Citation: Labat-de-Hoz, Leticia; Comas, Laura; Rubio-Ramos, Armando; Casares-Arias, Javier; Fernandez-Martin, Laura; Pantoja-Uceda, David; Martin, M. Teresa; Kremer, Leonor; Jimenez, M. Angeles; Correas, Isabel; Alonso, Miguel. "Structure and function of the N-terminal extension of the formin INF2"  Cell. Mol. Life Sci. 79, 571-571 (2022).
PubMed: 36306014

Assembly members:

Assembly members:
entity_1, polymer, 36 residues, 3795.17 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SVKEGAQRKWAALKEKLGPQ DSDPTEANLESADPEX

Data sets:
Data typeCount
13C chemical shifts112
1H chemical shifts235

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1INF2(2-36)1

Entities:

Entity 1, INF2(2-36) 36 residues - 3795.17 Da.

C-end amidated The C-terminal X corresponds to the C-terminal amide group

1   SERVALLYSGLUGLYALAGLNARGLYSTRP
2   ALAALALEULYSGLULYSLEUGLYPROGLN
3   ASPSERASPPROTHRGLUALAASNLEUGLU
4   SERALAASPPROGLUNH2

Samples:

sample_1: INF2(2-36) 1 mM; D2O, [U-2H], 7 % v/v; H2O 63 % v/v; DSS 0.1 mM; TFE, [U-98% 2H], 30 % v/v

sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1D 1Hsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection, processing

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE NEO 600 MHz MHz

Related Database Links:

NCBI NP_071934