BMRB Entry 51401

Name: SNAC-mEpoR-TM
Published: 2022-06-13

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51401

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

SNAC-mEpoR-TM

Deposition date:

2022-04-17

Original release date:

2022-06-13

Authors:

Mravic, Marco

Citation:

Citation: Mravic, Marco. "Spectra of mEpoR transmembrane fragment in 2H-DPC, monomeric state" .

Assembly members:

Assembly members:
entity_1, polymer, 33 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SHHWNDPLILTLSLILVLIS LLLTVLALLSHRR

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	117
15N chemical shifts	31
1H chemical shifts	241

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	mEpoR-TM_monomer	1

Entities:

Entity 1, mEpoR-TM_monomer 33 residues - Formula weight is not available

1

SER

HIS

TRP

ASN

ASP

PRO

LEU

ILE

LEU

2

THR

LEU

SER

LEU

ILE

LEU

VAL

LEU

ILE

SER

3

LEU

THR

VAL

LEU

ALA

LEU

SER

4

HIS

ARG

Samples:

sample_1: mEpoR TM, [U-95% 13C; U-95% 15N], 1 mM; D2O 5%; H2O 95%; sodium chloride 20 mM; EDTA 1 mM; sodium acetate, [U-2H], 50 mM; DPC, [U-99% 2H], 400 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 5.2; pressure: 1 atm; temperature: 318.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D (H)CCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1

Software:

SPARKY - chemical shift assignment

TOPSPIN v4.0.2 - collection

NMR spectrometers:

Bruker Avance 800 MHz

UNP	P14753
AlphaFold	Q63852