BMRB Entry 51400

Title:
1H, 13C, and 15N Chemical Shift Assignments for amyloidogenic SEM1(68-85) peptide
Deposition date:
2022-04-14
Original release date:
2022-04-20
Authors:
Blokhin, Dmitriy; Sanchugova, Daria; Klochkov, Vladimir
Citation:

Citation: Blokhin, Dmitriy; Sanchugova, Daria; Klochkov, Vladimir. "The structure of the fibril-forming SEM1(68-85) peptide increasing the HIV infection"  .

Assembly members:

Assembly members:
entity_1, polymer, 18 residues, 2193.2481 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Experimental source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: TYHVDANDHDQSRKSQQY

Data typeCount
13C chemical shifts64
15N chemical shifts18
1H chemical shifts91

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SEM1(68-85) peptide1

Entities:

Entity 1, SEM1(68-85) peptide 18 residues - 2193.2481 Da.

1   THRTYRHISVALASPALAASNASPHISASP
2   GLNSERARGLYSSERGLNGLNTYR

Samples:

sample_1: SEM1(68-85) peptide 0.9 mM

sample_conditions_1: pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQC/HMQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQC/HMQCsample_1isotropicsample_conditions_1
HMBC 13C (H_[C].Jmultibond)sample_1isotropicsample_conditions_1

Software:

CcpNmr Analysis v2.4 - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks