BMRB Entry 51395

Name: drosophila pAbp PABC
Published: 2023-07-04

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51395

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

drosophila pAbp PABC

Deposition date:

2022-04-07

Original release date:

2023-07-04

Authors:

Hollmann, Nele Merret; Simon, Bernd; Hennig, Janosch

Citation:

Citation: Hollmann, Nele Merret; Jagtap, Pravin; Linse, Johanna-Barbara; Ullmann, Philip; Payr, Marco; Murciano, Brice; Simon, Bernd; Hub, Jochen; Hennig, Janosch. "Upstream of N-Ras C-terminal cold shock domains mediate poly(A) specificity in a novel RNA recognition mode and bind poly(A) binding protein" Nucleic Acids Res. 51, 1895-1913 (2023).
PubMed: 36688322

Assembly members:

Assembly members:
entity_1, polymer, 85 residues, Formula weight is not available

Natural source:

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM-11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: KNSEKLIASLLANAKPQEQK QILGERLYPMIEHMHANLAG KITGMLLEIENSELLHMIED QEALKAKVEEAVAVLQVHRV TEPAN

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	144
15N chemical shifts	74
1H chemical shifts	74

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	drosophila pAbp PABC	1

Entities:

Entity 1, drosophila pAbp PABC 85 residues - Formula weight is not available

1

LYS

ASN

SER

GLU

LYS

LEU

ILE

ALA

SER

LEU

2

LEU

ALA

ASN

ALA

LYS

PRO

GLN

GLU

GLN

LYS

3

GLN

ILE

LEU

GLY

GLU

ARG

LEU

TYR

PRO

MET

4

ILE

GLU

HIS

MET

HIS

ALA

ASN

LEU

ALA

GLY

5

LYS

ILE

THR

GLY

MET

LEU

GLU

ILE

GLU

6

ASN

SER

GLU

LEU

HIS

MET

ILE

GLU

ASP

7

GLN

GLU

ALA

LEU

LYS

ALA

LYS

VAL

GLU

8

ALA

VAL

ALA

VAL

LEU

GLN

VAL

HIS

ARG

VAL

9

THR

GLU

PRO

ALA

ASN

Samples:

sample_1: pAbp PABC, [U-99% 13C; U-99% 15N], 0.3 mM; sodium azide 0.01%; sodium phosphate 50 mM; sodium chloride 50 mM; DTT 1 mM

sample_conditions_1: pH: 6.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3.5pl7 - collection

NMRPipe - processing

CARA - chemical shift assignment, peak picking

NMR spectrometers:

Bruker AVANCE III 600 MHz