BMRB Entry 51390
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51390
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Price, Caitlin; Hussain, Alya; Henen, Morkos; Vogeli, Beat; Sette, Marco; Barbato, Gaetano; Boelens, Rolf. "Histone-like nucleoid structuring protein interaction with DNA in Shigella flexneri" .
Assembly members:
entity_1, polymer, 48 residues, Formula weight is not available
Natural source: Common Name: Shigella flexneri Taxonomy ID: 623 Superkingdom: Bacteria Kingdom: not available Genus/species: Shigella flexneri
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: Pet28(a)+
Entity Sequences (FASTA):
entity_1: RAQRPAKYSYVDENGETKTW
TGQGRTPAVIKKAMDEQGKS
LDDFLIKQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 130 |
| 15N chemical shifts | 41 |
| 1H chemical shifts | 216 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HN-S CTD | 1 |
Entities:
Entity 1, HN-S CTD 48 residues - Formula weight is not available
| 1 | ARG | ALA | GLN | ARG | PRO | ALA | LYS | TYR | SER | TYR | ||||
| 2 | VAL | ASP | GLU | ASN | GLY | GLU | THR | LYS | THR | TRP | ||||
| 3 | THR | GLY | GLN | GLY | ARG | THR | PRO | ALA | VAL | ILE | ||||
| 4 | LYS | LYS | ALA | MET | ASP | GLU | GLN | GLY | LYS | SER | ||||
| 5 | LEU | ASP | ASP | PHE | LEU | ILE | LYS | GLN |
Samples:
sample_1: HN-S CTD, [U-100% 13C; U-100% 15N], 700 uM; NaCl 20 mM
sample_conditions_1: ionic strength: 30 mM; pH: 6.4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHANH | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR - chemical shift assignment, peak picking
TOPSPIN v4.1.1 - collection, data analysis
VNMR - collection
NMRDraw - data analysis, peak picking, processing
NMR spectrometers:
- Bruker AVANCE NEO 600 MHz
- Varian INOVA 900 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks