BMRB Entry 51384

Name: SH3-DNAse1L3-CTD
Published: 2022-04-13

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51384

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

SH3-DNAse1L3-CTD

Deposition date:

2022-04-02

Original release date:

2022-04-13

Authors:

Mccord, Jon; Masoumzadeh, Elahe; Latham, Michael; Sutton, Roger

Citation:

Citation: McCord, Jon; Engavale, Minal; Masoumzadeh, Elahe; Villarreal, Johanna; Mapp, Britney; Latham, Michael; Keyel, Peter; Sutton, R Bryan. "Structural features of Dnase1L3 responsible for serum antigen clearance" Commun. Biol. 5, 825-825 (2022).
PubMed: 35974043

Assembly members:

Assembly members:
entity_1, polymer, 109 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: purified from the natural source

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSHHHHHHTSNPWATAEYDY DAAEDNELTFVENDKIINIE FVDDDWWLGELEKDGSKGLF PSNYVSLGNGSGSENLYFQG GSYAMGSSRAFTNSKKSVTL RKKTKSKRS

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	272
15N chemical shifts	93
1H chemical shifts	93

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SH3-DNase1L3-CTD	1

Entities:

Entity 1, SH3-DNase1L3-CTD 109 residues - Formula weight is not available

1

MET

SER

HIS

THR

SER

2

ASN

PRO

TRP

ALA

THR

ALA

GLU

TYR

ASP

TYR

3

ASP

ALA

GLU

ASP

ASN

GLU

LEU

THR

PHE

4

VAL

GLU

ASN

ASP

LYS

ILE

ASN

ILE

GLU

5

PHE

VAL

ASP

TRP

LEU

GLY

GLU

6

LEU

GLU

LYS

ASP

GLY

SER

LYS

GLY

LEU

PHE

7

PRO

SER

ASN

TYR

VAL

SER

LEU

GLY

ASN

GLY

8

SER

GLY

SER

GLU

ASN

LEU

TYR

PHE

GLN

GLY

9

GLY

SER

TYR

ALA

MET

GLY

SER

ARG

ALA

10

PHE

THR

ASN

SER

LYS

SER

VAL

THR

LEU

11

ARG

LYS

THR

LYS

SER

LYS

ARG

SER

Samples:

sample_1: HEPES 20 mM; D2O 10%; sodium chloride 300 mM; SH3 DNase1L3_CTD, [U-100% 13C; U-100% 15N], 700 uM

sample_conditions_1: ionic strength: 0.3 M; pH: 7.4; pressure: 1 atm; temperature: 295 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe - data analysis

CcpNMR vCcpNmr Analysis Version 2.5 - chemical shift assignment

NMR spectrometers:

Agilent Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks