BMRB Entry 51381

Name: Backbone Chemical Shift Assignments of the S. cerevisiae Tom22(1-74)
Published: 2022-09-26

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51381

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone Chemical Shift Assignments of the S. cerevisiae Tom22(1-74)

Deposition date:

2022-03-25

Original release date:

2022-09-26

Authors:

Sucec, Iva; Schanda, Paul

Citation:

Citation: Sucec, Iva; Schanda, Paul. "Backbone Chemical Shift Assignments of the cytosolic domain of mitochondrial Tom22 receptor" .

Assembly members:

Assembly members:
entity_1, polymer, 78 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET47b-TEV-GB1-His6

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GHMVELTEIKDDVVQLDEPQ FSRNQAIVEEKASATNNDVV DDEDDSDSDFEDEFDENETL LDRIVALKDIVPPGKRLE

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
spectral_peak_list

Data type	Count
13C chemical shifts	194
15N chemical shifts	65
1H chemical shifts	65

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Tom22	1

Entities:

Entity 1, Tom22 78 residues - Formula weight is not available

Protein sequence in the assignment project includes two additional residues at the N-ter and 2 at the C-ter, however, the numbering was conserved to match the biological sequence; Tom22(1-74): MVELTEIKDDVVQLDEPQFSRNQAIVEEKASATNNDVVDDEDDSDSDFEDEFDENETLLDRIVALKDIVPPGKR.

1

GLY

HIS

MET

VAL

GLU

LEU

THR

GLU

ILE

LYS

2

ASP

VAL

GLN

LEU

ASP

GLU

PRO

GLN

3

PHE

SER

ARG

ASN

GLN

ALA

ILE

VAL

GLU

4

LYS

ALA

SER

ALA

THR

ASN

ASP

VAL

5

ASP

GLU

ASP

SER

ASP

SER

ASP

PHE

6

GLU

ASP

GLU

PHE

ASP

GLU

ASN

GLU

THR

LEU

7

LEU

ASP

ARG

ILE

VAL

ALA

LEU

LYS

ASP

ILE

8

VAL

PRO

GLY

LYS

ARG

LEU

GLU

Samples:

sample_1: Tom22(1-74), [U-99% 13C; U-99% 15N], 0.75 mM; H2O 55.5 M; D2O 10%; potassium phosphate 20 mM

sample_conditions_1: pH: 6; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N TROSY	sample_1	isotropic	sample_conditions_1
3D CBCANH	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D hNcocaNH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection, processing

CcpNMR v3 - chemical shift assignment, peak picking

CYANA - data analysis

TALOS+ - data analysis

NMR spectrometers:

Bruker Ascend 850 MHz
Bruker Ascend 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks