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BMRB Entry 51381 BMRB - Biological Magnetic Resonance Bank

BMRB Entry 51381

Title:
Backbone Chemical Shift Assignments of the S. cerevisiae Tom22(1-74)
Deposition date:
2022-03-25
Original release date:
2022-09-26
Authors:
Sucec, Iva; Schanda, Paul
Citation:

Citation: Sucec, Iva; Schanda, Paul. "Backbone Chemical Shift Assignments of the cytosolic domain of mitochondrial Tom22 receptor"  .

Assembly members:

Assembly members:
entity_1, polymer, 78 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET47b-TEV-GB1-His6

Entity Sequences (FASTA):

Data typeCount
13C chemical shifts194
15N chemical shifts65
1H chemical shifts65

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Tom221

Entities:

Entity 1, Tom22 78 residues - Formula weight is not available

Protein sequence in the assignment project includes two additional residues at the N-ter and 2 at the C-ter, however, the numbering was conserved to match the biological sequence; Tom22(1-74): MVELTEIKDDVVQLDEPQFSRNQAIVEEKASATNNDVVDDEDDSDSDFEDEFDENETLLDRIVALKDIVPPGKR.

1   GLYHISMETVALGLULEUTHRGLUILELYS
2   ASPASPVALVALGLNLEUASPGLUPROGLN
3   PHESERARGASNGLNALAILEVALGLUGLU
4   LYSALASERALATHRASNASNASPVALVAL
5   ASPASPGLUASPASPSERASPSERASPPHE
6   GLUASPGLUPHEASPGLUASNGLUTHRLEU
7   LEUASPARGILEVALALALEULYSASPILE
8   VALPROPROGLYLYSARGLEUGLU

Samples:

sample_1: Tom22(1-74), [U-99% 13C; U-99% 15N], 0.75 mM; H2O 55.5 M; D2O 10%; potassium phosphate 20 mM

sample_conditions_1: pH: 6; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
3D CBCANHsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D hNcocaNHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection, processing

CcpNMR v3 - chemical shift assignment, peak picking

CYANA - data analysis

TALOS+ - data analysis

NMR spectrometers:

  • Bruker Ascend 850 MHz
  • Bruker Ascend 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks