BMRB Entry 51364
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51364
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Moliner, Rafael; Girych, Mykhailo; Brunello, Cecilia; Kovaleva, Vera; Biojone, Caroline; Enkavi, Giray; Antenucci, Lina; Kot, Erik; Goncharuk, Sergey; Kaurinkoski, Katja; Kuutti, Mirjami; Fred, Senem; Elsila, Lauri; Sakson, Sven; Cannarozzo, Cecilia; Diniz, Cassiano; Seiffert, Nina; Rubiolo, Anna; Haapaniemi, Hele; Meshi, Elsa; Nagaeva, Elina; Ohman, Tiina; Rog, Tomasz; Kankuri, Esko; Vilar, Marcal; Varjosalo, Markku; Korpi, Esa; Permi, Perttu; Mineev, Konstantin; Saarma, Mart; Vattulainen, Ilpo; Casarotto, Plinio; Castren, Eero. "Psychedelics promote plasticity by directly binding to BDNF receptor TrkB" Nat. Neurosci. 26, 1032-1041 (2023).
Assembly members:
entity_1, polymer, 45 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: Escherichia coli Vector: pGEMEX1
Entity Sequences (FASTA):
entity_1: MTDKTGREHLSVYAVVVIAS
VVGFCLLVMLFLLKLARHSK
FGMKG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 195 |
| 15N chemical shifts | 45 |
| 1H chemical shifts | 338 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TrkB-TM, chain 1 | 1 |
| 2 | TrkB-TM, chain 2 | 1 |
Entities:
Entity 1, TrkB-TM, chain 1 45 residues - Formula weight is not available
| 1 | MET | THR | ASP | LYS | THR | GLY | ARG | GLU | HIS | LEU | ||||
| 2 | SER | VAL | TYR | ALA | VAL | VAL | VAL | ILE | ALA | SER | ||||
| 3 | VAL | VAL | GLY | PHE | CYS | LEU | LEU | VAL | MET | LEU | ||||
| 4 | PHE | LEU | LEU | LYS | LEU | ALA | ARG | HIS | SER | LYS | ||||
| 5 | PHE | GLY | MET | LYS | GLY |
Samples:
sample_1: TrkB-TM monomer, [U-13C; U-15N], 1.05 mM; D2O, [U-2H], 5%; DMPC, [U-2H], 9.6 mM; DHPC, [U-2H], 42.7 mM; NaN3 0.01%; sodium phosphate 20 mM; TCEP 1.4 mM
sample_conditions_1: ionic strength: 20 mM; pH: 5.98; pressure: 1 atm; temperature: 313 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N TROSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA - chemical shift assignment, data analysis, peak picking
NMR spectrometers:
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks