BMRB Entry 51362

Name: Structural basis for tRNA recognition and regulation of METTL1-WDR4 methyltransferase
Published: 2023-01-13

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51362

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structural basis for tRNA recognition and regulation of METTL1-WDR4 methyltransferase

Deposition date:

2022-03-14

Original release date:

2023-01-13

Authors:

Viennet, Thibault; Li, Jiazhi; Gregory, Richard; Arthanari, Haribabu

Citation:

Citation: Li, Jiazhi; Wang, Longfei; Hahn, Quentin; Nowak, Radoslaw; Viennet, Thibault; Oreliana, Esteban; Burman, Shourya; Yue, Hong; Hunkeler, Moritz; Fontana, Pietro; Wu, Hao; Arthanari, Haribabu; Fischer, Eric; Gregory, Richard. "Structural basis of regulated m7G tRNA modification by METTL1-WDR4" Nature 613, 391-397 (2023).
PubMed: 36599985

Assembly members:

Assembly members:
entity_1, polymer, 81 residues, 9426.37 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETDuet-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SQDPNSMAAETRNVAGAEAP PPQKRYYRQRAHSNPMADHT LRYPVKPEEMDWSELYPEFF APLTQNQSHDDPKDKKEKRA Q

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	193
15N chemical shifts	60
1H chemical shifts	60

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	METTL1 1-75	1

Entities:

Entity 1, METTL1 1-75 81 residues - 9426.37 Da.

1

SER

GLN

ASP

PRO

ASN

SER

MET

ALA

GLU

2

THR

ARG

ASN

VAL

ALA

GLY

ALA

GLU

ALA

PRO

3

PRO

GLN

LYS

ARG

TYR

ARG

GLN

ARG

4

ALA

HIS

SER

ASN

PRO

MET

ALA

ASP

HIS

THR

5

LEU

ARG

TYR

PRO

VAL

LYS

PRO

GLU

MET

6

ASP

TRP

SER

GLU

LEU

TYR

PRO

GLU

PHE

7

ALA

PRO

LEU

THR

GLN

ASN

GLN

SER

HIS

ASP

8

ASP

PRO

LYS

ASP

LYS

GLU

LYS

ARG

ALA

9

GLN

Samples:

sample_1: METTL1 1-75, [U-100% 13C; U-100% 15N], 420 mM; NaPi 50 mM; NaCl 100 mM; TCEP 1 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6; pressure: 1 atm; temperature: 288 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCOCA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR v2.4.2 - chemical shift assignment

NMR spectrometers:

Bruker AVANCE NEO 700 MHz