BMRB Entry 51361

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51361

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

1H, 13C and 15N Backbone Chemical Shift Assignments of the SH3 domain of the JNK-interacting protein 2, conformations corresponding to proline 664 cis-trans isomerization

Deposition date:

2022-03-14

Original release date:

2024-08-09

Authors:

Marino Perez, Laura; Ringkjobing Jensen, Malene

Citation:

Citation: Marino Perez, Laura; Ielasi, Francesco; Lee, Alexandra; Delaforge, Elise; Juyoux, Pauline; Tengo, Maud; Davis, Roger; Palencia, Andres; Jensen, Malene Ringkjobing. "Structural basis of homodimerization of the JNK scaffold protein JIP2 and its heterodimerization with JIP1" Structure ., .-. (2024).
PubMed: 39013462

Assembly members:

Assembly members:
entity_1, polymer, 63 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pESPRIT

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GRREQTHRAVFRFIPRHPDE LELDVDDPVLVEAEEDDFWF RGFNMRTGERGVFPAFYAHA VPG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
- assigned_chemical_shifts_2

Data type	Count
13C chemical shifts	346
15N chemical shifts	112
1H chemical shifts	112

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	WT JIP2-SH3, cis conformer	1
2	WT JIP2-SH3, trans conformer	1

Entities:

Entity 1, WT JIP2-SH3, cis conformer 63 residues - Formula weight is not available

1

GLY

ARG

GLU

GLN

THR

HIS

ARG

ALA

VAL

2

PHE

ARG

PHE

ILE

PRO

ARG

HIS

PRO

ASP

GLU

3

LEU

GLU

LEU

ASP

VAL

ASP

PRO

VAL

LEU

4

VAL

GLU

ALA

GLU

ASP

PHE

TRP

PHE

5

ARG

GLY

PHE

ASN

MET

ARG

THR

GLY

GLU

ARG

6

GLY

VAL

PHE

PRO

ALA

PHE

TYR

ALA

HIS

ALA

7

VAL

PRO

GLY

Samples:

sample_1: JIP2-SH3 WT, [U-95% 13C; U-95% 15N], 2 mM; NaCl 150 mM; HEPES 50 mM

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe - processing

NMRFAM-SPARKY - chemical shift assignment, data analysis, peak picking

TOPSPIN - collection

NMR spectrometers:

Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks