BMRB Entry 51360

Title:
Backbone resonance assignments of the C-terminal domain of Sam68
Deposition date:
2022-03-11
Original release date:
2022-11-23
Authors:
Malki, Idir; Dominguez, Cyril
Citation:

Citation: Malki, Idir; Liepina, Inara; Kogelnik, Nora; Watmuff, Hollie; Robinson, Sue; Lightfoot, Adam; Gonchar, Oksana; Bottrill, Andrew; Fry, Andrew; Dominguez, Cyril. "Cdk1-mediated threonine phosphorylation of Sam68 modulates its RNA binding, alternative splicing activity and cellular functions"  Nucleic Acids Res. 50, 13045-13062 (2022).
PubMed: 36537190

Assembly members:

Assembly members:
entity_1, polymer, 107 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6P-2

Data sets:
Data typeCount
13C chemical shifts101
15N chemical shifts53
1H chemical shifts53

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Sam68 C-terminal domain1

Entities:

Entity 1, Sam68 C-terminal domain 107 residues - Formula weight is not available

1   GLYPROLEUGLYSERGLNPHELEUGLULEU
2   SERTYRLEUASNGLYVALPROGLUPROSER
3   ARGGLYARGGLYVALPROVALARGGLYARG
4   GLYALAALAPROPROPROPROPROVALPRO
5   ARGGLYARGGLYVALGLYPROPROARGGLY
6   ALALEUVALARGGLYTHRPROVALARGGLY
7   ALAILETHRARGGLYALATHRVALTHRARG
8   GLYVALPROPROPROPROTHRVALARGGLY
9   ALAPROALAPROARGALAARGTHRALAGLY
10   ILEGLNARGILEPROLEUPROPROPROPRO
11   ALAPROGLUTHRTYRGLUGLU

Samples:

sample_1: C-terminal domain of Sam68, [U-99% 13C; U-99% 15N], 500 uM

sample_conditions_1: ionic strength: 0.25 M; pH: 7.0; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCACONHsample_1isotropicsample_conditions_1
3D CBCANHsample_1isotropicsample_conditions_1

Software:

CcpNMR v2.4.2 - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks