BMRB Entry 51360

Name: Backbone resonance assignments of the C-terminal domain of Sam68
Published: 2022-11-23

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51360

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone resonance assignments of the C-terminal domain of Sam68

Deposition date:

2022-03-11

Original release date:

2022-11-23

Authors:

Malki, Idir; Dominguez, Cyril

Citation:

Citation: Malki, Idir; Liepina, Inara; Kogelnik, Nora; Watmuff, Hollie; Robinson, Sue; Lightfoot, Adam; Gonchar, Oksana; Bottrill, Andrew; Fry, Andrew; Dominguez, Cyril. "Cdk1-mediated threonine phosphorylation of Sam68 modulates its RNA binding, alternative splicing activity and cellular functions" Nucleic Acids Res. 50, 13045-13062 (2022).
PubMed: 36537190

Assembly members:

Assembly members:
entity_1, polymer, 107 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-6P-2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GPLGSQFLELSYLNGVPEPS RGRGVPVRGRGAAPPPPPVP RGRGVGPPRGALVRGTPVRG AITRGATVTRGVPPPPTVRG APAPRARTAGIQRIPLPPPP APETYEE

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	101
15N chemical shifts	53
1H chemical shifts	53

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Sam68 C-terminal domain	1

Entities:

Entity 1, Sam68 C-terminal domain 107 residues - Formula weight is not available

1

GLY

PRO

LEU

GLY

SER

GLN

PHE

LEU

GLU

LEU

2

SER

TYR

LEU

ASN

GLY

VAL

PRO

GLU

PRO

SER

3

ARG

GLY

ARG

GLY

VAL

PRO

VAL

ARG

GLY

ARG

4

GLY

ALA

PRO

VAL

PRO

5

ARG

GLY

ARG

GLY

VAL

GLY

PRO

ARG

GLY

6

ALA

LEU

VAL

ARG

GLY

THR

PRO

VAL

ARG

GLY

7

ALA

ILE

THR

ARG

GLY

ALA

THR

VAL

THR

ARG

8

GLY

VAL

PRO

THR

VAL

ARG

GLY

9

ALA

PRO

ALA

PRO

ARG

ALA

ARG

THR

ALA

GLY

10

ILE

GLN

ARG

ILE

PRO

LEU

PRO

11

ALA

PRO

GLU

THR

TYR

GLU

Samples:

sample_1: C-terminal domain of Sam68, [U-99% 13C; U-99% 15N], 500 uM

sample_conditions_1: ionic strength: 0.25 M; pH: 7.0; pressure: 1 atm; temperature: 278 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCACONH	sample_1	isotropic	sample_conditions_1
3D CBCANH	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR v2.4.2 - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks