BMRB Entry 51357

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51357

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Title:
1H, 13C and 15N resonance assignments of TbBDF5-bromo1 domain from Trypanosoma brucei
Deposition date:
2022-03-10
Original release date:
2024-08-09
Authors:
Yang, Li; Zhang, Xiaole; Zhang, Jiahai; Tu, Xiaoming
Citation:

Citation: Yang, Li; Zhang, Xiaole; Zhang, Jiahai; Tu, Xiaoming. "1H, 13C and 15N resonance assignments of TbBDF5-bromo1 domain from Trypanosoma brucei"  Biomol. NMR Assign. 16, 253-255 (2022).
PubMed: 35652998

Assembly members:

Assembly members:
entity_1, polymer, 130 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Trypanosoma brucei   Taxonomy ID: 5691   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Trypanosoma brucei

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22(b)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSQNRQLLYPREEMVSLVRS LDRPQENGLFSQDVLLQYPE LAESYTKVCPNRCDLATAAD RAAKGAYGYDVQLTTLKEDI RLMVNNCILFNGAEGAYADA ARTFEKFAMGKIDAYISQKV GGLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts214
15N chemical shifts113
1H chemical shifts536

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TbBDF5-bromo11

Entities:

Entity 1, TbBDF5-bromo1 130 residues - Formula weight is not available

1   METSERGLNASNARGGLNLEULEUTYRPRO
2   ARGGLUGLUMETVALSERLEUVALARGSER
3   LEUASPARGPROGLNGLUASNGLYLEUPHE
4   SERGLNASPVALLEULEUGLNTYRPROGLU
5   LEUALAGLUSERTYRTHRLYSVALCYSPRO
6   ASNARGCYSASPLEUALATHRALAALAASP
7   ARGALAALALYSGLYALATYRGLYTYRASP
8   VALGLNLEUTHRTHRLEULYSGLUASPILE
9   ARGLEUMETVALASNASNCYSILELEUPHE
10   ASNGLYALAGLUGLYALATYRALAASPALA
11   ALAARGTHRPHEGLULYSPHEALAMETGLY
12   LYSILEASPALATYRILESERGLNLYSVAL
13   GLYGLYLEUGLUHISHISHISHISHISHIS

Samples:

sample_1: TbBDF5-bromo1, [U-99% 15N], 0.8 mM; H2O 90%; D2O 10%; NaH2PO4 25 mM; NaCl 100 mM; DTT 1 mM; EDTA 1 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.8; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

SPARKY - chemical shift assignment

NMR spectrometers:

  • Bruker DMX 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks