BMRB Entry 51355

Title:
Backbone and side-chain NMR assignment of the human TRPV3 ion channel N-terminal intrinsically disordered region
Deposition date:
2022-03-09
Original release date:
2022-06-06
Authors:
Wiedemann, Christoph; Goretzki, Benedikt; Merz, Zoe; Schmitt, Pauline; Tebbe, Frederike; Hellmich, Ute
Citation:

Citation: Wiedemann, Christoph; Goretzki, Benedikt; Merz, Zoe; Tebbe, Frederike; Schmitt, Pauline; Hellmich, Ute. "Extent of intrinsic disorder and NMR chemical shift assignments of the distal N-termini from human TRPV1, TRPV2 and TRPV3 ion channels"  Biomol. NMR Assignments 16, 289-296 (2022).
PubMed: 35666427

Assembly members:

Assembly members:
entity_1, polymer, 118 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Data sets:
Data typeCount
13C chemical shifts458
15N chemical shifts99
1H chemical shifts620

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hsTRPV3-IDR1

Entities:

Entity 1, hsTRPV3-IDR 118 residues - Formula weight is not available

1   LYSALAHISPROLYSGLUMETVALPROLEU
2   METGLYLYSARGVALALAALAPROSERGLY
3   ASNPROALAILELEUPROGLULYSARGPRO
4   ALAGLUILETHRPROTHRLYSLYSSERALA
5   HISPHEPHELEUGLUILEGLUGLYPHEGLU
6   PROASNPROTHRVALALALYSTHRSERPRO
7   PROVALPHESERLYSPROMETASPSERASN
8   ILEARGGLNCYSILESERGLYASNCYSASP
9   ASPMETASPSERPROGLNSERPROGLNASP
10   ASPVALTHRGLUTHRPROSERASNPROASN
11   SERPROSERALAGLNLEUALALYSGLUGLU
12   GLNARGARGLYSLYSARGARGLEU

Samples:

sample_1: hsTRPV3-IDR, [U-99% 13C; U-99% 15N], 300 uM; D2O, [U-99% 2H], 10%; DSS 0.1 mM; H2O 90%; sodium phosphate 10 mM; sodium chloride 300 mM; DTT 1 mM

sample_conditions_1: ionic strength: 0.31 M; pH: 6.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D (H)CC(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection, processing

CcpNMR - data analysis

NMR spectrometers:

  • Bruker AVANCE III 600 MHz

Related Database Links:

UNP Q8NET8-1
AlphaFold Q8NFH2

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks