BMRB Entry 51354

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51354

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Title:
Backbone NMR assignment of the human TRPV2 ion channel N-terminal intrinsically disordered region
Deposition date:
2022-03-09
Original release date:
2022-06-06
Authors:
Wiedemann, Christoph; Goretzki, Benedikt; Merz, Zoe; Hellmich, Ute
Citation:

Citation: Wiedemann, Christoph; Goretzki, Benedikt; Merz, Zoe; Tebbe, Frederike; Schmitt, Pauline; Hellmich, Ute. "Extent of intrinsic disorder and NMR chemical shift assignments of the distal N-termini from human TRPV1, TRPV2 and TRPV3 ion channels"  Biomol. NMR Assignments 16, 289-296 (2022).
PubMed: 35666427

Assembly members:

Assembly members:
hsTRPV2-IDR, polymer, 72 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hsTRPV2-IDR: TSPSSSPVFRLETLDGGQED GSEADRGKLDFGSGLPPMES QFQGEDRKFAPQIRVNLNYR KGTGASQPDPNR

Data sets:
Data typeCount
13C chemical shifts198
15N chemical shifts63
1H chemical shifts240

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hsTRPV2-IDR1

Entities:

Entity 1, hsTRPV2-IDR 72 residues - Formula weight is not available

1   THRSERPROSERSERSERPROVALPHEARG
2   LEUGLUTHRLEUASPGLYGLYGLNGLUASP
3   GLYSERGLUALAASPARGGLYLYSLEUASP
4   PHEGLYSERGLYLEUPROPROMETGLUSER
5   GLNPHEGLNGLYGLUASPARGLYSPHEALA
6   PROGLNILEARGVALASNLEUASNTYRARG
7   LYSGLYTHRGLYALASERGLNPROASPPRO
8   ASNARG

Samples:

sample_1: hsTRPV2-IDR, [U-99% 13C; U-99% 15N], 300 uM; D2O, [U-99% 2H], 10%; DSS 0.1 mM; H2O 90%; TRIS 10 mM; sodium chloride 100 mM

sample_conditions_1: ionic strength: 0.11 M; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1

Software:

CARA - chemical shift assignment

TOPSPIN - collection, processing

CcpNMR - data analysis

NMR spectrometers:

  • Bruker AVANCE III 600 MHz

Related Database Links:

UNP Q9Y5S1-1
AlphaFold Q9Y670

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks