BMRB Entry 51350

Title:
NPSL2
Deposition date:
2022-03-07
Original release date:
2022-03-31
Authors:
Liu, Yaping; Keane, Sarah
Citation:

Citation: Liu, Yaping; Munsayac, Aldrex; Hall, Ian; Keane, Sarah. "Solution structure of NPSL2, a regulatory element in the oncomiR-1 RNA"  J. Mol. Biol. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 43 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: In vitro transcription   Host organism: In vitro

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGGUGUAGAAAAGUAAGGGA AACUCAAACCCCUUUCUACA CCC

Data sets:
Data typeCount
13C chemical shifts39
1H chemical shifts231

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NPSL21

Entities:

Entity 1, NPSL2 43 residues - Formula weight is not available

1   GGGUGUAGAA
2   AAGUAAGGGA
3   AACUCAAACC
4   CCUUUCUACA
5   CCC

Samples:

sample_1: NPSL2 500 uM; potassium phosphate 50 mM

sample_2: NPSL2 500 uM; potassium phosphate 50 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 7.5; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

NMRFx Analyst - processing

NMRViewJ - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE NEO 600 MHz
  • Bruker AVANCE NEO 800 MHz