BMRB Entry 5134
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5134
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Gollmick, F.; Lorenz, M.; Dornberger, U.; von Langen, J.; Diekmann, S.; Fritzsche, H.. "Solution Structure of dAATAA and dAAUAA DNA Bulges" Nucleic Acids Res. 30, 2669-2677 (2002).
PubMed: 12060684
Assembly members:
5'-D(*GP*CP*AP*TP*CP*GP*AP*AP*UP*AP*AP*GP*CP*TP*AP*CP*G)-3', polymer, 17 residues, Formula weight is not available
5'-D(*CP*GP*TP*AP*GP*CP*CP*GP*AP*TP*GP*C)-3', polymer, 12 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
5'-D(*GP*CP*AP*TP*CP*GP*AP*AP*UP*AP*AP*GP*CP*TP*AP*CP*G)-3': GCATCGAAUAAGCTACG
5'-D(*CP*GP*TP*AP*GP*CP*CP*GP*AP*TP*GP*C)-3': CGTAGCCGATGC
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 248 |
| 31P chemical shifts | 27 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | 5'-D(*GP*CP*AP*TP*CP*GP*AP*AP*UP*AP*AP*GP*CP*TP*AP*CP*G)-3' | 1 |
| 2 | 5'-D(*CP*GP*TP*AP*GP*CP*CP*GP*AP*TP*GP*C)-3' | 2 |
Entities:
Entity 1, 5'-D(*GP*CP*AP*TP*CP*GP*AP*AP*UP*AP*AP*GP*CP*TP*AP*CP*G)-3' 17 residues - Formula weight is not available
| 1 | DG | DC | DA | DT | DC | DG | DA | DA | DU | DA | ||||
| 2 | DA | DG | DC | DT | DA | DC | DG |
Entity 2, 5'-D(*CP*GP*TP*AP*GP*CP*CP*GP*AP*TP*GP*C)-3' 12 residues - Formula weight is not available
| 1 | DC | DG | DT | DA | DG | DC | DC | DG | DA | DT | ||||
| 2 | DG | DC |
Samples:
sample_1: 5'-D(*GP*CP*AP*TP*CP*GP*AP*AP*UP*AP*AP*GP*CP*TP*AP*CP*G)-3' 3.8 mM; 5'-D(*CP*GP*TP*AP*GP*CP*CP*GP*AP*TP*GP*C)-3' 3.8 mM; phosphate buffer 10 mM; NaCl 100 mM; EDTA 0.05 mM; D2O 100%
sample_cond_1: ionic strength: 100 mM; pH: 7; pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | sample_1 | not available | sample_cond_1 |
| E-COSY | sample_1 | not available | sample_cond_1 |
| 31P-1H heteronuclear correlated | sample_1 | not available | sample_cond_1 |
Software:
NMRPipe v3 - processing
MARDIGRAS v3.2 - iterative matrix relaxation
DYANA v1.5 - structure solution
AMBER v5.0 - data analysis, refinement
NMR spectrometers:
- Varian INOVA 500 MHz
- Varian INOVA 600 MHz
- Varian INOVA 750 MHz