BMRB Entry 51337

Title:
5caC-containing dsDNA 12mers at three pH conditions
Deposition date:
2022-02-22
Original release date:
2022-02-28
Authors:
Dubini, Romeo Cosimo Arrigo; Korytiakova, Eva; Schinkel, Thea; Heinrichs, Pia; Carell, Thomas; Rovo, Petra
Citation:

Citation: Dubini, Romeo Cosimo Arrigo; Korytiakova, Eva; Schinkel, Thea; Heinrichs, Pia; Carell, Thomas; Rovo, Petra. "1H NMR Chemical Exchange Techniques Reveal Local and Global Effects of Oxidized Cytosine Derivatives"  ACS Phys. Chem. Au. 2, 237-246 (2022).
PubMed: 35637781

Assembly members:

Assembly members:
entity_1, polymer, 12 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GCGATXGATCGC

Data typeCount
13C chemical shifts34
15N chemical shifts15
1H chemical shifts248

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
15caC dsDNA1

Entities:

Entity 1, 5caC dsDNA 12 residues - Formula weight is not available

1   DGDCDGDADT1CCDGDADTDC
2   DGDC

Samples:

sample_1: 5caC-modified 12mer dsDNA 0.66 mM

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 310 K

sample_conditions_2: pH: 5.8; pressure: 1 atm; temperature: 310 K

sample_conditions_3: pH: 4.7; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_2
2D 1H-15N HSQCsample_1isotropicsample_conditions_2
2D 1H-1H NOESYsample_1isotropicsample_conditions_2
2D 1H-13C HSQCsample_1isotropicsample_conditions_3
2D 1H-15N HSQCsample_1isotropicsample_conditions_3
2D 1H-1H NOESYsample_1isotropicsample_conditions_3

Software:

NMRFAM-SPARKY v3.11 - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 800 MHz