BMRB Entry 51334

Name: MDM2AD
Published: 2022-10-11

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51334

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

MDM2AD

Deposition date:

2022-02-20

Original release date:

2022-10-11

Authors:

Song, Qinyan; Rainey, Jan; Liu, Paul

Citation:

Citation: Song, Qinyan; Liu, Xiang-Qin; Rainey, Jan. "The MDMX acidic domain competes with the p53 transactivation domain for MDM2 N-terminal domain binding" Biochim. Biophys. Acta Mol. Cell Res. 1869, 119319-119319 (2022).
PubMed: 35780910

Assembly members:

Assembly members:
entity_1, polymer, 86 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-32

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: STGTPSNPDLDAGVSEHSGD WLDQDSVSDQFSVEFEVESL DSEDYSLSEEGQELSDEDDE VYQVTVYQAGESDTDSFEED PEISLA

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
heteronucl_NOEs
- heteronucl_NOEs_1

Data type	Count
13C chemical shifts	247
15N chemical shifts	81
1H chemical shifts	81
heteronuclear NOE values	83

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MDM2	1

Entities:

Entity 1, MDM2 86 residues - Formula weight is not available

1

SER

THR

GLY

THR

PRO

SER

ASN

PRO

ASP

LEU

2

ASP

ALA

GLY

VAL

SER

GLU

HIS

SER

GLY

ASP

3

TRP

LEU

ASP

GLN

ASP

SER

VAL

SER

ASP

GLN

4

PHE

SER

VAL

GLU

PHE

GLU

VAL

GLU

SER

LEU

5

ASP

SER

GLU

ASP

TYR

SER

LEU

SER

GLU

6

GLY

GLN

GLU

LEU

SER

ASP

GLU

ASP

GLU

7

VAL

TYR

GLN

VAL

THR

VAL

TYR

GLN

ALA

GLY

8

GLU

SER

ASP

THR

ASP

SER

PHE

GLU

ASP

9

PRO

GLU

ILE

SER

LEU

ALA

Samples:

sample_1: MDM2AD, [U-95% 13C; U-95% 15N], 800 uM; D2O, [U-99% 2H], 10%; H2O 90%; DSS 1 mM; sodium azide 0.05 % w/v; sodium chloride 40 mM; sodium phosphate 20 mM

sample_conditions_1: ionic strength: 82 mM; pH: 7.0; pressure: 1 atm; temperature: 293K K

Experiments:

Name	Sample	Sample state	Sample conditions
1D 1H	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR - chemical shift assignment, data analysis, peak picking

NMRPipe - processing

VNMRJ v4.2A - collection

NMR spectrometers:

Varian INOVA 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks