BMRB Entry 51325

Title:
N-terminal domain of SARS-CoV-2 Nsp8 protein
Deposition date:
2022-02-12
Original release date:
2022-02-21
Authors:
Trevino, Miguel; Pantoja-Uceda, David; Laurents, Douglas; Mompean, Miguel
Citation:

Citation: Trevino, Miguel; Pantoja-Uceda, David; Laurents, Douglas; Mompean, Miguel. "SARS-CoV-2 Nsp8 N-terminal domain folds autonomously and binds dsRNA"  Nucleic Acids Res. 51, 10041-10048 (2023).
PubMed: 37665006

Assembly members:

Assembly members:
entity_1, polymer, 84 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: SARS-CoV-2   Taxonomy ID: 2697049   Superkingdom: Viruses   Kingdom: not available   Genus/species: Betacoronavirus HCoV-SARS

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts306
15N chemical shifts80
1H chemical shifts534
T1 relaxation values79
T1rho relaxation values79
coupling constants64
heteronuclear NOE values79

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Nsp8 NTD, chain 11
2Nsp8 NTD, chain 21

Entities:

Entity 1, Nsp8 NTD, chain 1 84 residues - Formula weight is not available

1   ALAILEALASERGLUPHESERSERLEUPRO
2   SERTYRALAALAPHEALATHRALAGLNGLU
3   ALATYRGLUGLNALAVALALAASNGLYASP
4   SERGLUVALVALLEULYSLYSLEULYSLYS
5   SERLEUASNVALALALYSSERGLUPHEASP
6   ARGASPALAALAMETGLNARGLYSLEUGLU
7   LYSMETALAASPGLNALAMETTHRGLNMET
8   TYRLYSGLNALAARGSERGLUASPLYSARG
9   ALALYSVALTHR

Samples:

sample_1: molecule_1, [U-100% 13C; U-100% 15N], 100 uM; NaCl 50 mM; KH2PO4 10 mM

sample_conditions_1: ionic strength: 60 mM; pH: 6.1; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
hsqct1etf3gpsisample_1isotropicsample_conditions_1
hsqctref3gpsisample_1isotropicsample_conditions_1
hsqcnoef3gpsisample_1isotropicsample_conditions_1
hnhagp3dsample_1isotropicsample_conditions_1

Software:

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE NEO 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks