BMRB Entry 5132

Title:
1H and 15N assignments of ATTm+4, a truncated version of ATT, an trypsin/chymotrypsin inhibitor from Arabidopsis thaliana
Deposition date:
2001-09-06
Original release date:
2003-06-10
Authors:
Zhao, Qin; Chae, Young-Kee; Markley, John
Citation:

Citation: Zhao, Qin; Chae, Young-Kee; Markley, John. "NMR Solution Structure of ATTp, an Arabidopsis thaliana Trypsin Inhibitor"  Biochemistry 41, 12284-12296 (2002).
PubMed: 12369816

Assembly members:

Assembly members:
attm+4, polymer, 66 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Pichia pastoris   Vector: pPIC9k/attp

Entity Sequences (FASTA):

Data sets:
Data typeCount
15N chemical shifts55
1H chemical shifts219

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1attm+41

Entities:

Entity 1, attm+4 66 residues - Formula weight is not available

1   TYRVALGLUPHEGLNGLYASNGLUCYSLEU
2   LYSGLUTYRGLYGLYASPVALGLYPHEGLY
3   PHECYSALAPROARGILEPHEPROTHRILE
4   CYSTYRTHRARGCYSARGGLUASNLYSGLY
5   ALALYSGLYGLYARGCYSARGTRPGLYGLN
6   GLYSERASNVALLYSCYSLEUCYSASPPHE
7   CYSGLYASPTHRPROGLN

Samples:

sample_1: attm+4, [U-15N], 1 mM; sodium acetate, [U-2H], 50 mM; DSS 0.1 mM

Ex-cond: pH: 5.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N HSQCnot availablenot availablenot available
15N TOCSYnot availablenot availablenot available
15N NOESYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker DMX 500 MHz

Related Database Links:

BMRB 5133

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks