BMRB Entry 51319

Name: phosphomimetic variant of the C-terminal domain of the b2-adrenergic receptor
Published: 2024-08-09

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51319

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

phosphomimetic variant of the C-terminal domain of the b2-adrenergic receptor

Deposition date:

2022-02-09

Original release date:

2024-08-09

Authors:

Guillien, Myriam; Sibille, Nathalie

Citation:

Citation: Guillien, Myriam; Mouhand, Assia; Sagar, Amin; Fournet, Aurelie; Allemand, Frederic; Pereira, Glaecia; Thureau, Aurelien; Bernado, Pau; Baneres, Jean-Louis; Sibille, Nathalie. "Phosphorylation motif dictates GPCR C-terminal domain conformation and arrestin interaction" Structure 31, 1394-1406 (2023).
PubMed: 37669668

Assembly members:

Assembly members:
entity_1, polymer, 76 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET1a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAMALRRSSLKAYGNGYEEN GNEGEQEGYHVEQEKENKLL CEDLPGTEDFVGHQGTVPED NIDEQGRNCETNDELL

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	216
15N chemical shifts	72
1H chemical shifts	72

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	pm-b2AR-Cter GPCR	1

Entities:

Entity 1, pm-b2AR-Cter GPCR 76 residues - Formula weight is not available

1

GLY

ALA

MET

ALA

LEU

ARG

SER

LEU

2

LYS

ALA

TYR

GLY

ASN

GLY

TYR

GLU

ASN

3

GLY

ASN

GLU

GLY

GLU

GLN

GLU

GLY

TYR

HIS

4

VAL

GLU

GLN

GLU

LYS

GLU

ASN

LYS

LEU

5

CYS

GLU

ASP

LEU

PRO

GLY

THR

GLU

ASP

PHE

6

VAL

GLY

HIS

GLN

GLY

THR

VAL

PRO

GLU

ASP

7

ASN

ILE

ASP

GLU

GLN

GLY

ARG

ASN

CYS

GLU

8

THR

ASN

ASP

GLU

LEU

Samples:

sample_1: pm-b2AR-Cter GPCR, [U-13C; U-15N], 206 uM; Bis-Tris 50 mM; NaCl 150 mM; EDTA 1 mM; TCEP 0.5 mM; DSS-d6, [U-2H], 5 mM

sample_conditions_1: ionic strength: 150 mM; pH: 6.7; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
2D HSQC	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection

NMR spectrometers:

Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks