BMRB Entry 51317

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51317

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Title:
C-terminal domain of the Growth Hormone Secretagogue Receptor type 1a
Deposition date:
2022-02-09
Original release date:
2022-06-08
Authors:
Guillien, Myriam; Sibille, Nathalie
Citation:

Citation: Guillien, Myriam; Mouhand, Assia; Fournet, Aurelie; Gontier, Amandine; Marti Navia, Aleix; Cordeiro, Tiago; Allemand, Frederic; Thureau, Aurelien; Baneres, Jean-Louis; Bernado, Pau; Sibille, Nathalie. "Structural Insights into the Intrinsically Disordered GPCR C-Terminal Region, Major Actor in Arrestin-GPCR Interaction"  Biomolecules 12, 617-617 (2022).
PubMed: 35625550

Assembly members:

Assembly members:
entity_1, polymer, 32 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETM33

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GPMAGFEPFSQRKLSTLKDE SSRAWTESSINT

Data sets:
Data typeCount
13C chemical shifts83
15N chemical shifts29
1H chemical shifts29

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GHSR-Cter GPCR1

Entities:

Entity 1, GHSR-Cter GPCR 32 residues - Formula weight is not available

1   GLYPROMETALAGLYPHEGLUPROPHESER
2   GLNARGLYSLEUSERTHRLEULYSASPGLU
3   SERSERARGALATRPTHRGLUSERSERILE
4   ASNTHR

Samples:

sample_1: wt-GHSR-Cter, [U-13C; U-15N], 312 uM; Bis-Tris 50 mM; NaCl 150 mM; EDTA 1 mM; TCEP 0.5 mM; DSS-d6, [U-2H], 5 mM

sample_conditions_1: ionic strength: 150 mM; pH: 6.7; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
2D HSQCsample_1isotropicsample_conditions_1

Software:

TOPSPIN - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks